Protein Variants | Comment | Organism |
---|---|---|
C188S | the activity of the mutant is much lower than that of the native enzyme | Bordetella bronchiseptica |
G74C | the activity of the mutant is much lower than that of the native enzyme | Bordetella bronchiseptica |
G74C/C188G | the mutant has a 5.6fold increase in activity compared with the G74C/C188S mutant | Bordetella bronchiseptica |
G74C/C188S | the mutant has 18000fold reduced activity compared to the wild type enzyme, the (S)-selective arylmalonate decarboxylase variant has a 220fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (99% ee) | Bordetella bronchiseptica |
G74C/M159L/C188G | the mutant shows a 210fold increase in activity compared with the G74C/C188S mutant | Bordetella bronchiseptica |
Y48F/G74C/C188G | the mutant shows a 23fold increase in activity compared with the G74C/C188S mutant | Bordetella bronchiseptica |
Y48F/G74C/M159L/C188G | the mutant shows a 920fold activity increase relative to the G74C/C188S mutant | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
2-phenylmalonate | mutant enzyme G74C/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
4.6 | - |
2-phenylmalonate | mutant enzyme Y48F/G74C/M159L/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
5.1 | - |
2-phenylmalonate | wild type enzyme, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
5.9 | - |
2-phenylmalonate | mutant enzyme G74C/M159L/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
7.1 | - |
2-phenylmalonate | mutant enzyme Y48F/G74C/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
7.5 | - |
2-phenylmalonate | mutant enzyme G74C/C188S, pH and temperature not specified in the publication | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | Q05115 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6-methoxynaphthalen-2-yl)(methyl)propanedioic acid | - |
Bordetella bronchiseptica | (R)-2-(6-methoxynaphthalen-2-yl)-2-methylpropanoic acid + CO2 | - |
? | |
2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | 2-arylpropionate + CO2 | - |
? | |
2-phenylmalonate | - |
Bordetella bronchiseptica | 2-phenylacetate + CO2 | - |
? | |
methyl(naphthalen-2-yl)propanedioic acid | - |
Bordetella bronchiseptica | (R)-2-methyl-2-(naphthalen-2-yl)propanoic acid + CO2 | - |
? | |
methyl(phenyl)propanedioic acid | - |
Bordetella bronchiseptica | (2R)-2-phenylpropanoic acid + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-phenylmalonate | mutant enzyme G74C/C188S, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.05 | - |
2-phenylmalonate | mutant enzyme G74C/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.44 | - |
2-phenylmalonate | mutant enzyme Y48F/G74C/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
3.3 | - |
2-phenylmalonate | mutant enzyme G74C/M159L/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
11 | - |
2-phenylmalonate | mutant enzyme Y48F/G74C/M159L/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
260 | - |
2-phenylmalonate | wild type enzyme, pH and temperature not specified in the publication | Bordetella bronchiseptica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
2-phenylmalonate | mutant enzyme G74C/C188S, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.015 | - |
2-phenylmalonate | mutant enzyme G74C/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.062 | - |
2-phenylmalonate | mutant enzyme Y48F/G74C/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.56 | - |
2-phenylmalonate | mutant enzyme G74C/M159L/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
2.5 | - |
2-phenylmalonate | mutant enzyme Y48F/G74C/M159L/C188G, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
50 | - |
2-phenylmalonate | wild type enzyme, pH and temperature not specified in the publication | Bordetella bronchiseptica |