Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli strain DH5alpha-MCR | Bordetella bronchiseptica |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, crystallized with ammonium sulfate under alkaline pH conditions with the aim of understanding the mechanism of the enantioselective reaction | Bordetella bronchiseptica |
Protein Variants | Comment | Organism |
---|---|---|
G74C/C188S | mutated AMDase produces arylpropionate of the opposite enantiomorph to that of the wild-type enzyme, although the enzymatic reaction proceeds with a slower rate than that of the wild type | Bordetella bronchiseptica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24740 | - |
electrospray ion mass spectrometry | Bordetella bronchiseptica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aryl-2-methylmalonate | Bordetella bronchiseptica | - |
2-arylpropionate + CO2 | optically active | ? | |
2-aryl-2-methylmalonate | Bordetella bronchiseptica KU1201 | - |
2-arylpropionate + CO2 | optically active | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | Q05115 | - |
- |
Bordetella bronchiseptica KU1201 | Q05115 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | 2-arylpropionate + CO2 | optically active | ? | |
2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica KU1201 | 2-arylpropionate + CO2 | optically active | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Bordetella bronchiseptica |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Arylmalonate decarboxylase | - |
Bordetella bronchiseptica |