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Literature summary for 4.1.1.76 extracted from

  • Miyamoto, K.; Yatake, Y.; Tamura, K.; Terao, Y.; Ohta, H.
    Purification and characterization of arylmalonate decarboxylase from Achromobacter sp. KU1311 (2007), J. Biosci. Bioeng., 104, 263-267.
    View publication on PubMed
Search for more literature for 4.1.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Achromobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 SDS-PAGE Achromobacter sp.
24740
-
 calculated from amino acid sequence Achromobacter sp.

Organism

Organism UniProt Comment Textmining
Achromobacter sp.
-
-
-
Achromobacter sp. KU1311
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, DEAE-Toyopearl column chromatography, and Butyl-Toyopearl column chromatography Achromobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.72
-
 crude cell extract Achromobacter sp.
380
-
 after 528fold purification Achromobacter sp.

Storage Stability

Storage Stability Organism
4°C, 100 mM Tris-HCl pH 8.0, 2 months, 50% loss of activity Achromobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl(phenyl)propanedioate
-
 Achromobacter sp. (2R)-2-phenylpropanoate + CO2
-
 ?
methyl(phenyl)propanedioate
-
 Achromobacter sp. KU1311 (2R)-2-phenylpropanoate + CO2
-
 ?
methyl(thiophen-2-yl)propanedioate
-
 Achromobacter sp. (2S)-2-thiophen-2-ylpropanoate + CO2
-
 ?
methyl(thiophen-2-yl)propanedioate
-
 Achromobacter sp. KU1311 (2S)-2-thiophen-2-ylpropanoate + CO2
-
 ?
additional information not active with (2S)-2-methyl-2-phenylbutanoic acid Achromobacter sp. ?
-
 ?
additional information not active with (2S)-2-methyl-2-phenylbutanoic acid Achromobacter sp. KU1311 ?
-
 ?
Phenylmalonate phenylmalonate is phenylpropanedioate Achromobacter sp. Phenylacetate + CO2
-
 ?
Phenylmalonate phenylmalonate is phenylpropanedioate Achromobacter sp. KU1311 Phenylacetate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
AMDase
-
 Achromobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Achromobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 activity decreases to 70% of the maximum at 25°C and 50°C Achromobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 retains 100% activity at 30°C, activity is almost negligible at 60°C Achromobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Achromobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Achromobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
 activity markedly decreases above pH 8.5 Achromobacter sp.