Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Bordetella bronchiseptica |
Protein Variants | Comment | Organism |
---|---|---|
G74C/C188S | very low activity | Bordetella bronchiseptica |
S36N | activity is about 1/10 compared to that of the wild-type enzyme | Bordetella bronchiseptica |
S36N/G74C/C188S | 10fold increased activity than the G74C/C188S double mutant enzyme | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
0.52 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
1.11 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
1.25 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
3.51 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
5.03 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
12.5 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
14.3 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
diethylamino ethanol-Toyopearl column chromatography and Butyl-Toyopearl column chromatography | Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-methyl-alpha-(alpha-thienyl)malonate | - |
Bordetella bronchiseptica | alpha-(alpha-thienyl)propionate + CO2 | - |
? | |
alpha-methyl-alpha-(beta-naphthyl)malonate | - |
Bordetella bronchiseptica | alpha-(beta-naphthyl) propionate + CO2 | - |
? | |
Phenylmalonate | - |
Bordetella bronchiseptica | Phenylacetate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
0.34 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
1.14 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
3.09 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
3.7 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
18.9 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
30.9 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica | |
200 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C | Bordetella bronchiseptica |