General Stability | Organism |
---|---|
ammonium sulfate stabilizes | Enterobacter cloacae |
thiamine diphosphate stabilizes | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
4-nitro-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
0.019 | - |
4-bromo-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
0.02 | - |
Indole-3-pyruvate | pH 6.5, 30°C | Enterobacter cloacae | |
0.048 | - |
4-chloro-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
0.111 | - |
4-ethyl-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
0.127 | - |
4-methyl-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
0.62 | - |
4-fluoro-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
1.05 | - |
4-methoxy-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
1.65 | - |
benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
3.38 | - |
pyruvate | pH 6.5, 30°C | Enterobacter cloacae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Enterobacter cloacae |
245000 | - |
gel filtration | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Enterobacter cloacae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | mechanism | Enterobacter cloacae |
Storage Stability | Organism |
---|---|
quite stable at 40°C without further additions | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(indol-3-yl)pyruvate | - |
Enterobacter cloacae | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
4-bromo-benzoylformic acid | - |
Enterobacter cloacae | 4-bromobenzaldehyde + CO2 | - |
? | |
4-chloro-benzoylformic acid | - |
Enterobacter cloacae | 4-chlorobenzaldehyde + CO2 | - |
? | |
4-ethyl-benzoylformic acid | - |
Enterobacter cloacae | 4-ethylbenzaldehyde + CO2 | - |
? | |
4-fluoro-benzoylformic acid | - |
Enterobacter cloacae | 4-fluorobenzaldehyde + CO2 | - |
? | |
4-methoxy-benzoylformic acid | - |
Enterobacter cloacae | 4-methoxybenzaldehyde + CO2 | - |
? | |
4-methyl-benzoylformic acid | - |
Enterobacter cloacae | 4-methylbenzaldehyde + CO2 | - |
? | |
4-nitro-benzoylformic acid | - |
Enterobacter cloacae | 4-nitrobenzaldehyde + CO2 | - |
? | |
benzoylformic acid | - |
Enterobacter cloacae | benzaldehyde + CO2 | - |
? | |
pyruvate | - |
Enterobacter cloacae | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | at pH-values between pH 5.6 and 6.0 the tetrameric form dominates, at pH values above 8.0, the monomeric form is present | Enterobacter cloacae |
tetramer | 4 * 60000, SDS-PAGE | Enterobacter cloacae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Enterobacter cloacae |