Protein Variants | Comment | Organism |
---|---|---|
Q487A | mutation diminishes the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
Q487G | mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
Q487I | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
Q487L | loss of acetolactate synthase activity, decrease in decarboxylase activity | Bacillus subtilis |
Q487S | mutation diminishes only the decarboxylase activity but maintains the acetolactate synthase activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
154 | - |
2-oxoisovalerate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
175 | - |
2-oxoisovalerate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
186 | - |
2-oxoisovalerate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
300 | - |
2-oxoisovalerate | wild-type, pH 7.0, 37°C | Bacillus subtilis | |
323 | - |
2-oxoisovalerate | mutant Q487I, pH 7.0, 37°C | Bacillus subtilis | |
342 | - |
2-oxoisovalerate | mutant Q487L, pH 7.0, 37°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q04789 | cf. EC 2.2.1.6 | - |
Bacillus subtilis 168 | Q04789 | cf. EC 2.2.1.6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate | - |
Bacillus subtilis | isobutanal + CO2 | - |
? | |
2-oxoisovalerate | - |
Bacillus subtilis 168 | isobutanal + CO2 | - |
? | |
additional information | acetolactate synthase AlsS, EC 2.2.1.6, is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis | ? | - |
? | |
additional information | acetolactate synthase AlsS, EC 2.2.1.6, is able to catalyze the decarboxylation of 2-oxoisovalerate both in vivo and in vitro | Bacillus subtilis 168 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlsS | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
2-oxoisovalerate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
0.8 | - |
2-oxoisovalerate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
1.1 | - |
2-oxoisovalerate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
4.8 | - |
2-oxoisovalerate | mutant Q487I, pH 7.0, 37°C | Bacillus subtilis | |
5.4 | - |
2-oxoisovalerate | mutant Q487L, pH 7.0, 37°C | Bacillus subtilis | |
8.9 | - |
2-oxoisovalerate | wild-type, pH 7.0, 37°C | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
2-oxoisovalerate | mutant Q487G, pH 7.0, 37°C | Bacillus subtilis | |
0.005 | - |
2-oxoisovalerate | mutant Q487S, pH 7.0, 37°C | Bacillus subtilis | |
0.006 | - |
2-oxoisovalerate | mutant Q487A, pH 7.0, 37°C | Bacillus subtilis | |
0.01 | - |
2-oxoisovalerate | mutant Q487I, pH 7.0, 37°C | Bacillus subtilis | |
0.02 | - |
2-oxoisovalerate | mutant Q487L, pH 7.0, 37°C | Bacillus subtilis | |
0.03 | - |
2-oxoisovalerate | wild-type, pH 7.0, 37°C | Bacillus subtilis |