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Literature summary for 4.1.1.72 extracted from
- Biosynthesis of branched-chain fatty acids in Bacillus subtilis. A decarboxylase is essential for branched-chain fatty acid synthetase (1988), J. Biol. Chem., 263, 18386-18396.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
L-3-Methyl-2-oxopentanoate | and 2-oxoisopentanoate, 2-oxoisohexanoate, value below, 30°C, pH 7.5 | Bacillus subtilis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| ferricyanide | stimulates | Bacillus subtilis | |
| additional information | no requirement for MgCl2 | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
| 39800 | - |
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
| 175000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methyl-2-oxopentanoate | Bacillus subtilis | - |
CO2 + 2-methylbutanal | - |
? | |
| additional information | Bacillus subtilis | essential for the synthesis of branched-chain fatty acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
30°C, pH 7.5 | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate | 5% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | CO2 + succinate semialdehyde | - |
? | |
| 2-oxoisohexanoate | 38% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | CO2 + isopentanal | - |
? | |
| 2-oxoisopentanoate | 63% of the activity with L-3-methyl- | Bacillus subtilis | CO2 + isobutanal | - |
? | |
| 3-methyl-2-oxopentanoate | - |
Bacillus subtilis | CO2 + 2-methylbutanal | - |
? | |
| L-3-methyl-2-oxopentanoate | stereospecificity towards the L-isomer | Bacillus subtilis | CO2 + 2-methylbutanal | - |
? | |
| additional information | essential for the synthesis of branched-chain fatty acids | Bacillus subtilis | ? | - |
? | |
| pyruvate | 25% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | ethanal + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6: about 60% of maximum activity, pH 9: about 55% of maximum activity | Bacillus subtilis |
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