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Literature summary for 4.1.1.71 extracted from
- Characterization and molecular properties of 2-oxoglutarate decarboxylase from Euglena gracilis (1991), Arch. Biochem. Biophys., 288, 22-28.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | stimulates | Euglena gracilis |  |
| DTT | stimulates | Euglena gracilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Euglena gracilis | |
| Hg2+ | - |
Euglena gracilis | |
| additional information | not: NaN3 | Euglena gracilis | |
| NEM | - |
Euglena gracilis | |
| Ni2+ | - |
Euglena gracilis | |
| PCMB | - |
Euglena gracilis | |
| Succinic semialdehyde | - |
Euglena gracilis | |
| Zn2+ | - |
Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
2-oxoglutarate | pH 7.0 | Euglena gracilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect | Euglena gracilis | |
| Co2+ | no effect | Euglena gracilis | |
| MgCl2 | stimulates | Euglena gracilis | |
| MgCl2 | Km: 0.093 mM | Euglena gracilis | |
| Mn2+ | stimulates | Euglena gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
4 * 62000, SDS-PAGE | Euglena gracilis |
| 250000 | - |
gel filtration | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate | highly specific | Euglena gracilis | succinate semialdehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 62000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42.5 | - |
- |
Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57.5 | - |
10 min, stable up to | Euglena gracilis |
| 62 | - |
pH 6.2-8.5, 10 min, stable | Euglena gracilis |
| 67 | - |
10 min, complete loss of activity | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Euglena gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: 65% of activity maximum, pH 9.0: 55% of activity maximum | Euglena gracilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.5 | 62°C, 10 min, stable | Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | slight stimulation | Euglena gracilis | |
| NADP+ | stimulates | Euglena gracilis | |
| NADPH | stimulates | Euglena gracilis | |
| thiamine diphosphate | required | Euglena gracilis | |
| thiamine diphosphate | Km: 0.056 mM | Euglena gracilis | |
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