Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain SG13009 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | covalent immobilization and stabilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic nanoparticles by a three-step immobilization/stabilization procedure at pH 8.0, 20°C, method development and evaluation, overview. The maximum enzyme loading is found to be 5.2 mg/g support in 3.5 mg/ml initial enzyme concentration at ionic strength of 1.25 M MgSO4. The covalently-bound enzyme is characterized in terms of its activity and stability for the formation of (S)-2-hydroxypropiophenone. The immobilized biocatalyst retains 95% of its original activity after five reaction cycles | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthesis of (S)-2-hydroxy-1-phenylpropanone by the immobilized recombinant enzyme is effective in cross acyloin reaction of benzaldehyde with acetaldehyde. The immobilized and free enzymes shows similar substrate specificity and activity | Pseudomonas putida | ? | - |
? | |
phenylglyoxylate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, substrate phenylglyoxylate | Pseudomonas putida |
8.5 | - |
assay at, cross acyloin reaction of benzaldehyde with acetaldehyde | Pseudomonas putida |