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Literature summary for 4.1.1.7 extracted from

  • Pan, X.X.; Li, J.J.; Wang, M.G.; He, W.S.; Jia, C.S.; Zhang, X.M.; Feng, B.; Li, D.L.; Zeng, Z.
    Oxidative decarboxylation of mandelic acid derivative by recombinant Escherichia coli: a novel method of ethyl vanillin synthesis (2013), Biotechnol. Lett., 35, 921-927.
    View publication on PubMed
Search for more literature for 4.1.1.7

Application

Application Comment Organism
synthesis the enzyme recombinantly expressed in Escherichia coli is useful for production of ethyl vanillin, i.e. 3-ethoxy-4-hydroxybenzaldehyde, which is a good substitute for vanillin in foods and perfumes since it is 3.5times stronger in flavor and keeps better condition in storage and transport Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
gene mdlC, recombinant expression in Escherichia coli strain BL21(DE3), suncloning in Escherichia coli strain DH5alpha Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information biotransformation using recycled resting Escherichia coli cells expressing the enzyme, the product yield is above 42% after four repeated cycles Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-ethoxy-4-hydroxybenzoylformate Pseudomonas putida
-
 ethyl vanillin + CO2
-
 ?
3-ethoxy-4-hydroxybenzoylformate Pseudomonas putida ATCC 12633
-
 ethyl vanillin + CO2
-
 ?
additional information Pseudomonas putida when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview ?
-
 ?
additional information Pseudomonas putida ATCC 12633 when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview ?
-
 ?
phenylglyoxylate Pseudomonas putida
-
 benzaldehyde + CO2
-
 ?
phenylglyoxylate Pseudomonas putida ATCC 12633
-
 benzaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20906 gene mdlC
-
Pseudomonas putida ATCC 12633 P20906 gene mdlC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-ethoxy-4-hydroxybenzoylformate
-
 Pseudomonas putida ethyl vanillin + CO2
-
 ?
3-ethoxy-4-hydroxybenzoylformate
-
 Pseudomonas putida ATCC 12633 ethyl vanillin + CO2
-
 ?
additional information when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview Pseudomonas putida ?
-
 ?
additional information when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview Pseudomonas putida ATCC 12633 ?
-
 ?
phenylglyoxylate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
phenylglyoxylate
-
 Pseudomonas putida ATCC 12633 benzaldehyde + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas putida