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Literature summary for 4.1.1.7 extracted from

  • Topal, K.; Atilgan, C.; Demir, A.; Aviyente, V.
    Understanding the mode of action of ThDP in benzoylformate decarboxylase (2010), Biopolymers, 93, 32-46.
    View publication on PubMed
Search for more literature for 4.1.1.7

Protein Variants

Protein Variants Comment Organism
H281A inactive Pseudomonas putida
H281F the mutation leads to a 5fold decrease in the enzyme activity Pseudomonas putida
H70A inactive Pseudomonas putida
H70L the mutant shows 30% decrease in the enzyme activity Pseudomonas putida
H70T the mutant shows 30% decrease in the enzyme activity Pseudomonas putida
L461A the mutant has decreased activity compared to the wild type enzyme, the enantioselectivity of the (S)-2-hydroxypropiophenone formation is increased by 6%. In the case of propionaldehyde, the enzyme activity increases considerably from 6% with wild type BFD to 21.5%. With butyraldehyde the already low enzyme activity does not change Pseudomonas putida
L461G the mutant has decreased activity compared to the wild type enzyme, the enantioselectivity of the (S)-2-hydroxypropiophenone formation is increased by 6%. In the case of propionaldehyde, the enzyme activity increases considerably from 6% with wild type BFD to 23%. With butyraldehyde the already low enzyme activity does not change Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains Mg2+ Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20906
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + acetaldehyde
-
 Pseudomonas putida (S)-2-hydroxypropiophenone i.e. acyloin ?
benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
butyraldehyde
-
 Pseudomonas putida ?
-
 ?
propionaldehyde
-
 Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
BFD
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Pseudomonas putida