Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
H281A | mutant with 152fold decreased kcat value compared to the wild type enzyme | Pseudomonas putida |
H281F | mutant with 4.9fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
H281N | mutant with 17fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
H281Q | mutant with 37fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
H281T | mutant with 159fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
H281W | mutant with 19fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
H281Y | mutant with 46fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
H70A | mutant exhibits 4000fold decreased catalytic activity compared to the wild type enzyme | Pseudomonas putida |
H70F | mutant exhibits a 236fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
H70L | mutant exhibits a 33fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
H70S | mutant exhibits a 197fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
H70T | mutant exhibits a 25fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
S26A | mutant with 21fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
S26L | mutant exhibits no significant loss of activity compared to the wild type enzyme (2fold decrease in kcat value) | Pseudomonas putida |
S26M | mutant exhibits no significant loss of activity compared to the wild type enzyme (12fold decrease in kcat value) | Pseudomonas putida |
S26T | mutant exhibits no significant loss of activity compared to the wild type enzyme (3fold decrease in kcat value) | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
benzoylformate | wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.29 | - |
benzoylformate | mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.29 | - |
benzoylformate | mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.38 | - |
benzoylformate | mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.49 | - |
benzoylformate | mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.54 | - |
benzoylformate | mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.8 | - |
benzoylformate | mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.85 | - |
benzoylformate | mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.85 | - |
benzoylformate | mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.89 | - |
benzoylformate | mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.9 | - |
benzoylformate | mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
0.94 | - |
benzoylformate | mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
1.2 | - |
benzoylformate | mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
1.2 | - |
benzoylformate | mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
1.5 | - |
benzoylformate | mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
2.9 | - |
benzoylformate | mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
7.8 | - |
benzoylformate | mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFDC | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
benzoylformate | mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
2 | - |
benzoylformate | mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
2.1 | - |
benzoylformate | mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.5 | - |
benzoylformate | mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
5.6 | - |
benzoylformate | mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
6.9 | - |
benzoylformate | mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
8.6 | - |
benzoylformate | mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
14 | - |
benzoylformate | mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
15 | - |
benzoylformate | mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
17 | - |
benzoylformate | mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
19 | - |
benzoylformate | mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
26 | - |
benzoylformate | mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
42 | - |
benzoylformate | mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
65 | - |
benzoylformate | mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
94 | - |
benzoylformate | mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
132 | - |
benzoylformate | mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
320 | - |
benzoylformate | wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | 0.5 mM, required for activity | Pseudomonas putida |