KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
2-aminoisobutanoate | activated by K+ | Burkholderia cepacia | |
2.2 | - |
2-aminoisobutanoate | activated by Rb+ | Burkholderia cepacia | |
4.4 | - |
2-aminoisobutanoate | activated by NH4+ | Burkholderia cepacia | |
5.8 | - |
2-aminoisobutanoate | activated by Mg2+ | Burkholderia cepacia | |
6.6 | - |
2-aminoisobutanoate | activated by Tl+ | Burkholderia cepacia | |
7.3 | - |
2-aminoisobutanoate | activated by Cs+ | Burkholderia cepacia | |
11 | - |
2-aminoisobutanoate | activated by Li+ | Burkholderia cepacia | |
13 | - |
2-aminoisobutanoate | activated by Na+ | Burkholderia cepacia | |
18 | - |
2-aminoisobutanoate | activated by Ca2+ | Burkholderia cepacia | |
66 | - |
2-aminoisobutanoate | activated by Ba2+ | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | gives 10fold lower activity compared to monovalent alkali cations of similar ionic radius, increases association of pyridoxal 5'-phosphate and decreases dissociation rate constant of pyridoxal 5'-phosphate | Burkholderia cepacia | |
Ca2+ | gives 10fold lower activity compared to monovalent alkali cations of similar ionic radius | Burkholderia cepacia | |
Cs+ | activates to a lesser extent than K+ | Burkholderia cepacia | |
K+ | activity is dependent on cations. K+ is the monovalent cation with the optimal size for catalytic activity, increases association of pyridoxal 5'-phosphate and decreases dissociation rate constant of pyridoxal 5'-phosphate | Burkholderia cepacia | |
Li+ | activates to a lesser extent than K+ | Burkholderia cepacia | |
Mg2+ | gives 10fold lower activity compared to monovalent alkali cations of similar ionic radius | Burkholderia cepacia | |
Na+ | activates to a lesser extent than K+, increases association of pyridoxal 5'-phosphate and decreases dissociation rate constant of pyridoxal 5'-phosphate | Burkholderia cepacia | |
NH4+ | activates to a lesser extent than K+ | Burkholderia cepacia | |
Rb+ | activates to a lesser extent than K+ | Burkholderia cepacia | |
Tl+ | activates to a lesser extent than K+ | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Aminoisobutanoate + pyruvate | - |
Burkholderia cepacia | L-Ala + acetone + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
2-aminoisobutanoate | activated by Ca2+ | Burkholderia cepacia | |
0.076 | - |
2-aminoisobutanoate | activated by Mg2+ | Burkholderia cepacia | |
0.86 | - |
2-aminoisobutanoate | activated by Li+ | Burkholderia cepacia | |
1.4 | - |
2-aminoisobutanoate | activated by Ba2+ | Burkholderia cepacia | |
1.83 | - |
2-aminoisobutanoate | activated by Na+ | Burkholderia cepacia | |
3.8 | - |
2-aminoisobutanoate | activated by Cs+ | Burkholderia cepacia | |
4.9 | - |
2-aminoisobutanoate | activated by NH4+ | Burkholderia cepacia | |
5.22 | - |
2-aminoisobutanoate | activated by Tl+ | Burkholderia cepacia | |
7.4 | - |
2-aminoisobutanoate | activated by Rb+ | Burkholderia cepacia | |
10.4 | - |
2-aminoisobutanoate | activated by K+ | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Burkholderia cepacia |