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Literature summary for 4.1.1.59 extracted from

  • Meier, A.; Worch, S.; Boer, E.; Hartmann, A.; Mascher, M.; Marzec, M.; Scholz, U.; Riechen, J.; Baronian, K.; Schauer, F.; Bode, R.; Kunze, G.
    Agdc1p - a gallic acid decarboxylase involved in the degradation of tannic acid in the yeast Blastobotrys (Arxula) adeninivorans (2017), Front. Microbiol., 8, 1777 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.59

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid 1 mM, 107% of initial activity Blastobotrys adeninivorans
dithiothreitol 1 mM, 116% of initial activity Blastobotrys adeninivorans
EDTA 1 mM, 146% of initial activity Blastobotrys adeninivorans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 73% loss of activity Blastobotrys adeninivorans
Cu2+ 1 mM, 96% loss of activity Blastobotrys adeninivorans
Fe2+ 1 mM, complete loss of activity Blastobotrys adeninivorans
Fe3+ 1 mM, 76% loss of activity Blastobotrys adeninivorans
Ni2+ 1 mM, complete loss of activity Blastobotrys adeninivorans
thiosulfate 1 mM, 15% loss of activity Blastobotrys adeninivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 gallic acid pH 6.2, 40°C Blastobotrys adeninivorans
3.2
-
 protocatechuic acid pH 6.2, 40°C Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27100
-
 gel filtration Blastobotrys adeninivorans

Organism

Organism UniProt Comment Textmining
Blastobotrys adeninivorans A0A060TAG5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
 pH 6.2, 40°C Blastobotrys adeninivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gallic acid
-
 Blastobotrys adeninivorans pyrogallol + CO2
-
 ?
additional information no substrtaes: 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid Blastobotrys adeninivorans ?
-
 ?
protocatechuic acid
-
 Blastobotrys adeninivorans catechol + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 27300, calculated from sequence, x * 25000, SDS-PAGE, recombinant protein Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Blastobotrys adeninivorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 more than 80% of maximum activity Blastobotrys adeninivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 stable within Blastobotrys adeninivorans
35
-
 24 h, 60% loss of activity Blastobotrys adeninivorans
40
-
 stable for 7 h, rapid loss of activity thereafter Blastobotrys adeninivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42
-
 gallic acid pH 6.2, 40°C Blastobotrys adeninivorans
44
-
 protocatechuic acid pH 6.2, 40°C Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Blastobotrys adeninivorans

pH Range

pH Minimum pH Maximum Comment Organism
5.6 7.1 more than 80% of maximum activity Blastobotrys adeninivorans

Expression

Organism Comment Expression
Blastobotrys adeninivorans expression is induced by gallic acid and protocatechuic acid up

General Information

General Information Comment Organism
physiological function a gene deletion mutant is not able to grow on medium with gallic acid as carbon source but can grow in presence of protocatechuic acid Blastobotrys adeninivorans