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Literature summary for 4.1.1.50 extracted from

  • Feng, J.; Wu, J.; Dai, N.; Lin, S.; Xu, H.H.; Deng, Z.; He, X.
    Discovery and characterization of BlsE, a radical S-adenosyl-L-methionine decarboxylase involved in the blasticidin S biosynthetic pathway (2013), PLoS ONE, 8, e68545.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Streptomyces griseochromogenes

Protein Variants

Protein Variants Comment Organism
M37F the mutant shows reduced catalytic efficiency compared to the wild type enzyme Streptomyces griseochromogenes
M37W the mutant shows reduced catalytic efficiency compared to the wild type enzyme Streptomyces griseochromogenes
M37Y the mutant shows reduced catalytic efficiency compared to the wild type enzyme Streptomyces griseochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00193
-
 S-adenosyl-L-methionine wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
0.00196
-
 S-adenosyl-L-methionine mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
0.00204
-
 S-adenosyl-L-methionine mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
0.00215
-
 S-adenosyl-L-methionine mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40200
-
 gel filtration Streptomyces griseochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine Streptomyces griseochromogenes
-
 S-adenosyl 3-(methylthio)propylamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces griseochromogenes Q841K9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Streptomyces griseochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
 Streptomyces griseochromogenes S-adenosyl 3-(methylthio)propylamine + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40200, His6-tagged enzyme, SDS-PAGE Streptomyces griseochromogenes

Synonyms

Synonyms Comment Organism
BlsE
-
 Streptomyces griseochromogenes
S-Adenosyl-L-methionine decarboxylase
-
 Streptomyces griseochromogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.021
-
 S-adenosyl-L-methionine mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
0.024
-
 S-adenosyl-L-methionine mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
0.024
-
 S-adenosyl-L-methionine mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
0.027
-
 S-adenosyl-L-methionine wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center the fully reconstituted His6-tagged enzyme contains approximately 6.8 iron equivalents per monomer Streptomyces griseochromogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
630
-
 S-adenosyl-L-methionine mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
680
-
 S-adenosyl-L-methionine mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
700
-
 S-adenosyl-L-methionine mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes
840
-
 S-adenosyl-L-methionine wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C Streptomyces griseochromogenes