Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis strain WB600 | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
K52D | the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme | Staphylococcus aureus |
K52E | the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme | Staphylococcus aureus |
N43D | the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme | Staphylococcus aureus |
N43D/K52D | the mutant shows dramatically improved acidic stability with prolonged half-life of 3.5 h (compared to the wild type of 1.5 h) at pH 4.0 | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0177 | - |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate | at pH 6.0 and 45°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-hydroxy-2-methyl-3-oxobutanoate | Staphylococcus aureus | - |
(3R)-3-hydroxybutan-2-one + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Staphylococcus aureus | (3R)-3-hydroxybutan-2-one + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
ALDC | - |
Staphylococcus aureus |
alpha-Acetolactate decarboxylase | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Staphylococcus aureus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | more than 60% activity between 30 and 50°C | Staphylococcus aureus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme activity is nearly 90% at -20°C and about 80% from 0 to 35°C after 10 h, while rapid inactivation is observed over 45°C | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Staphylococcus aureus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | the wild type enzyme has a half-life of 1.5 h at pH 4.0. The enzyme retains more than 75 % activity at pH 7.0-9.0 after preincubation for 10 h, while the enzyme activity at pH 6.0 is 41% after 10 h and decreases much more rapidly at pH 5.0 after 2.5 h | Staphylococcus aureus |