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Literature summary for 4.1.1.49 extracted from

  • Yevenes, A.; Gonzalez-Nilo, F.D.; Cardemil, E.
    Relevance of phenylalanine 216 in the affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase for Mn(II) (2007), Protein J., 26, 135-141.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli and in in the PEP carboxykinase-deficient Saccharomyces cerevisiae-strain PUK-3B Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F216Y Km: 0.075 (MnADP-), 0.323 (phosphoenolpyruvate), 17 (HCO3-), 0.063 (Mn2+). Mutation Phe216Tyr shows that the introduction of a hydroxyl group in the lateral chain of the residue produces a substantial loss in the enzyme affinity for Mn2+, suggesting an increase of the pKa of Lys213 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
Mn2+
-
Saccharomyces cerevisiae
0.034
-
ADP ADP in form of MnADP- Saccharomyces cerevisiae
0.063
-
Mn2+ mutant F216Y Saccharomyces cerevisiae
0.075
-
ADP mutant F216Y Saccharomyces cerevisiae
0.075
-
ADP ADP in form of MnADP- Saccharomyces cerevisiae
0.307
-
phosphoenolpyruvate
-
Saccharomyces cerevisiae
0.323
-
phosphoenolpyruvate mutant F216Y Saccharomyces cerevisiae
17
-
CO2 mutant F216Y Saccharomyces cerevisiae
17
-
CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
18
-
CO2 CO2 in form of HCO3- Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60980
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2
-
Saccharomyces cerevisiae ATP + oxaloacetate
-
r
ATP + oxaloacetate
-
Saccharomyces cerevisiae ADP + phosphoenolpyruvate + CO2
-
r

Synonyms

Synonyms Comment Organism
PEP carboxykinase
-
Saccharomyces cerevisiae
phosphoenolpyruvate carboxykinase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
assay at Saccharomyces cerevisiae