Literature summary for 4.1.1.49 extracted from

Salvarrey, M.S.; Cannata, J.J.B.; Cazzulo, J.J.
Phosphoenolpyruvate carboxykinase from the moderate halophile Vibrio costicola (1989), Biochem. J., 260, 221-230.

Search for more literature for 4.1.1.49

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	activates	Salinivibrio costicola

General Stability

General Stability	Organism
glycerol, 20% v/v, stabilizes against thermal inactivation at 30°C	Salinivibrio costicola

Inhibitors

Inhibitors	Comment	Organism	Structure
Fluorescein mercuric acetate	0.0008 mM, 50% inhibition	Salinivibrio costicola
iodoacetamide	less than 10% inhibition	Salinivibrio costicola
NEM	less than 10% inhibition	Salinivibrio costicola
o-Iodobenzoate	0.2 mM , 50% inhibition	Salinivibrio costicola
p-chloromercuribenzenesulfonate	0.0025 mM, 50% inhibition	Salinivibrio costicola
p-chloromercuribenzoate	0.007 mM, 50% inhibition	Salinivibrio costicola

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
17	-	CO2	CO2 in form of HCO3-	Salinivibrio costicola

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	gel filtration	Salinivibrio costicola
60000	-	1 * 60000, SDS-PAGE	Salinivibrio costicola

Organism

Organism	UniProt	Comment	Textmining
Salinivibrio costicola	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Salinivibrio costicola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Salinivibrio costicola

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphoenolpyruvate + CO2	-	Salinivibrio costicola	ATP + oxaloacetate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 60000, SDS-PAGE	Salinivibrio costicola

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	remaining activity after 214 h: 57% in presence of 20% v/v glycerol, 37% in presence of 1 mM NaCl, 31% in presence of 1 mM KCl, 28% in absence of salts	Salinivibrio costicola

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0008	-	Fluorescein mercuric acetate	-	Salinivibrio costicola
0.0025	-	p-chloromercuribenzenesulfonate	-	Salinivibrio costicola
0.007	-	p-chloromercuribenzoate	-	Salinivibrio costicola
0.2	-	o-Iodobenzoate	-	Salinivibrio costicola

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Release 2023.1 (January 2023)