Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.49 extracted from

  • Hunt, M.; Köhler, P.
    Purification and characterization of phosphoenolpyruvate carboxykinase from Trypanosoma brucei (1995), Biochim. Biophys. Acta, 1249, 15-22.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate slight activation of decarboxylation Trypanosoma brucei
fumarate slight activation of decarboxylation Trypanosoma brucei
malate slight activation of decarboxylation Trypanosoma brucei
succinate slight activation of decarboxylation Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
ATP 5 mM, inhibition of carboxylation Trypanosoma brucei
quinolinic acid 1 mM, complete inhibition of decarboxylation Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0103
-
ATP
-
Trypanosoma brucei
0.037
-
oxaloacetate
-
Trypanosoma brucei
0.04
-
ADP
-
Trypanosoma brucei
0.49
-
phosphoenolpyruvate
-
Trypanosoma brucei
26
-
CO2 CO2 in form of HCO3- Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma brucei 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dual cation requirement. Mn2+ is necessary to activate, and Mn2+ or Mg2+ forms the cation-nucleotide complex as the active form of the substrate Trypanosoma brucei
Mn2+ Km: 0.97 mM Trypanosoma brucei
Mn2+ dual cation requirement. Mn2+ is necessary to activate, and Mn2+ or Mg2+ forms the cation-nucleotide complex as the active form of the substrate Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
2 * 59000, SDS-PAGE Trypanosoma brucei
115000
-
gel filtration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei under physiological conditions the enzyme is bidirectional and is regulated primarily by mass action ?
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trypanosoma brucei

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell
-
Trypanosoma brucei
-
culture condition:ethanol-grown cell
-
Trypanosoma brucei
-
procyclic form
-
Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81.8
-
-
Trypanosoma brucei

Storage Stability

Storage Stability Organism
-80°C, stable for 3 months Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2
-
Trypanosoma brucei ATP + oxaloacetate
-
?
ATP + oxaloacetate
-
Trypanosoma brucei ADP + phosphoenolpyruvate + CO2
-
?
additional information under physiological conditions the enzyme is bidirectional and is regulated primarily by mass action Trypanosoma brucei ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 59000, SDS-PAGE Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
carboxylation Trypanosoma brucei
7.3
-
decarboxylation Trypanosoma brucei