Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | slight activation of decarboxylation | Trypanosoma brucei | |
fumarate | slight activation of decarboxylation | Trypanosoma brucei | |
malate | slight activation of decarboxylation | Trypanosoma brucei | |
succinate | slight activation of decarboxylation | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 5 mM, inhibition of carboxylation | Trypanosoma brucei | |
quinolinic acid | 1 mM, complete inhibition of decarboxylation | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
ATP | - |
Trypanosoma brucei | |
0.037 | - |
oxaloacetate | - |
Trypanosoma brucei | |
0.04 | - |
ADP | - |
Trypanosoma brucei | |
0.49 | - |
phosphoenolpyruvate | - |
Trypanosoma brucei | |
26 | - |
CO2 | CO2 in form of HCO3- | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dual cation requirement. Mn2+ is necessary to activate, and Mn2+ or Mg2+ forms the cation-nucleotide complex as the active form of the substrate | Trypanosoma brucei | |
Mn2+ | Km: 0.97 mM | Trypanosoma brucei | |
Mn2+ | dual cation requirement. Mn2+ is necessary to activate, and Mn2+ or Mg2+ forms the cation-nucleotide complex as the active form of the substrate | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, SDS-PAGE | Trypanosoma brucei |
115000 | - |
gel filtration | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | under physiological conditions the enzyme is bidirectional and is regulated primarily by mass action | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | - |
Trypanosoma brucei | - |
culture condition:ethanol-grown cell | - |
Trypanosoma brucei | - |
procyclic form | - |
Trypanosoma brucei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
81.8 | - |
- |
Trypanosoma brucei |
Storage Stability | Organism |
---|---|
-80°C, stable for 3 months | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | - |
Trypanosoma brucei | ATP + oxaloacetate | - |
? | |
ATP + oxaloacetate | - |
Trypanosoma brucei | ADP + phosphoenolpyruvate + CO2 | - |
? | |
additional information | under physiological conditions the enzyme is bidirectional and is regulated primarily by mass action | Trypanosoma brucei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59000, SDS-PAGE | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
carboxylation | Trypanosoma brucei |
7.3 | - |
decarboxylation | Trypanosoma brucei |