Crystallization (Comment) | Organism |
---|---|
molecular dynamics simulations of wild-type at 25°C in the apo form and in the presence of the substrate | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
F246S | mutation at C-terminus, increases flexibility | Saccharolobus solfataricus |
G212E | mutation interferes with phosphate binding | Saccharolobus solfataricus |
L236Q | mutation destabilizes helix alpha8' | Saccharolobus solfataricus |
M237T | mutation destabilizes helix alpha8' | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | wild-type, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.000105 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | wild-type, pH 7.5, 60°C | Saccharolobus solfataricus | |
0.0013 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant F246S, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.0029 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant M237T, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.0034 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant L236Q, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.0217 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant G212E, pH 7.5, 25°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q06121 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Saccharolobus solfataricus | 1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? | |
additional information | a different degree of loop mobility correlates with different rate-limiting steps in the catalytic mechanism. Both the application of activating mutations and temperature increase lead to a net increase in the catalytic turnover number due to acceleration of the product release rate relative to the chemical transformation steps | Saccharolobus solfataricus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | wild-type, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.16 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant G212E, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.2 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant L236Q, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.23 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant M237T, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.32 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant F246S, pH 7.5, 25°C | Saccharolobus solfataricus | |
2.1 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | wild-type, pH 7.5, 60°C | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant G212E, pH 7.5, 25°C | Saccharolobus solfataricus | |
60 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant L236Q, pH 7.5, 25°C | Saccharolobus solfataricus | |
80 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant M237T, pH 7.5, 25°C | Saccharolobus solfataricus | |
240 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | mutant F246S, pH 7.5, 25°C | Saccharolobus solfataricus | |
1180 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | wild-type, pH 7.5, 25°C | Saccharolobus solfataricus | |
20000 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | wild-type, pH 7.5, 60°C | Saccharolobus solfataricus |