Application | Comment | Organism |
---|---|---|
drug development | indole-3-glycerol phosphate synthase of Mycobacterium tuberculosis is an attractive target for new anti-Tuberculosis drug development | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) STAR | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, pH 7.5 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel-filtration chromatography | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Mycobacterium tuberculosis | - |
1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatographic purification, Phenyl Sepharose High Sub FF column, ultrafiltration membrane (molecular weight cutoff) of 10,000 Da, Sephacryl S-100 column | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Mycobacterium tuberculosis | 1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000 | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
IGPS | - |
Mycobacterium tuberculosis |
indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis |
MtIGPS | - |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | activation energy value of 8.4 kcal/mol, the linearity of the Arrhenius plot demonstrates that there is no change in the rate-limiting step in the temperature range employed | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, pH 7.5 | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, pH 7.5 | Mycobacterium tuberculosis |