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Literature summary for 4.1.1.48 extracted from

  • Czekster, C.M.; Neto, B.A.; Lapis, A.A.; Dupont, J.; Santos, D.S.; Basso, L.A.
    Steady-state kinetics of indole-3-glycerol phosphate synthase from Mycobacterium tuberculosis (2009), Arch. Biochem. Biophys., 486, 19-26.
    View publication on PubMed

Application

Application Comment Organism
drug development indole-3-glycerol phosphate synthase of Mycobacterium tuberculosis is an attractive target for new anti-Tuberculosis drug development Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) STAR Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, pH 7.5 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel-filtration chromatography Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Mycobacterium tuberculosis
-
1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatographic purification, Phenyl Sepharose High Sub FF column, ultrafiltration membrane (molecular weight cutoff) of 10,000 Da, Sephacryl S-100 column Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Mycobacterium tuberculosis 1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
IGPS
-
Mycobacterium tuberculosis
indole-3-glycerol phosphate synthase
-
Mycobacterium tuberculosis
MtIGPS
-
Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 35 activation energy value of 8.4 kcal/mol, the linearity of the Arrhenius plot demonstrates that there is no change in the rate-limiting step in the temperature range employed Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, pH 7.5 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.9
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, pH 7.5 Mycobacterium tuberculosis