Application | Comment | Organism |
---|---|---|
medicine | IGPS may be a potential target for the development of anti-tuberculosis drugs | Mycobacterium tuberculosis |
medicine | IGPS may be a potential target for the development of anti-tuberculosis drugs | Mycobacterium tuberculosis variant bovis BCG |
medicine | IGPS may be a potential target for the development of anti-tuberculosis drugs | Mycobacterium tuberculosis H37Ra |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 | Mycobacterium tuberculosis |
expressed in Escherichia coli strain BL21 | Mycobacterium tuberculosis H37Ra |
Protein Variants | Comment | Organism |
---|---|---|
E168A | site directed mutagenesis | Mycobacterium tuberculosis |
E168A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
H170A | site directed mutagenesis | Mycobacterium tuberculosis |
H170A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
L193A | site directed mutagenesis | Mycobacterium tuberculosis |
L193A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
L196A | site directed mutagenesis | Mycobacterium tuberculosis |
L196A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
additional information | site directed mutagenesis studies indicate that ATB107 may inhibit IGPS activity by reducing the binding affinity for substrate of residues Glu168 and Asn189 | Mycobacterium tuberculosis |
additional information | site directed mutagenesis studies indicate that ATB107 may inhibit IGPS activity by reducing the binding affinity for substrate of residues Glu168 and Asn189 | Mycobacterium tuberculosis H37Ra |
N189A | site directed mutagenesis | Mycobacterium tuberculosis |
N189A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
N192A | site directed mutagenesis | Mycobacterium tuberculosis |
N192A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
P63A | site directed mutagenesis | Mycobacterium tuberculosis |
P63A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
R191A | site directed mutagenesis | Mycobacterium tuberculosis |
R191A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
S64A | site directed mutagenesis | Mycobacterium tuberculosis |
S64A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
V169A | site directed mutagenesis | Mycobacterium tuberculosis |
V169A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATB107 | 1-azabicyclo[2.2.2]octan-3-one[4-(phenylamino)-6-(1-piperidinyl)-1,3,5-triazin-2-yl]hydrazone, 0.1 microg/ml minimum inhibitory concentration, potent competitive inhibitor of the substrate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | Mycobacterium tuberculosis | |
ATB107 | 1-azabicyclo[2.2.2]octan-3-one[4-(phenylamino)-6-(1-piperidinyl)-1,3,5-triazin-2-yl]hydrazone, 0.1 microg/ml minimum inhibitory concentration, potent competitive inhibitor of the substrate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | Mycobacterium tuberculosis H37Ra | |
ATB107 | 1-azabicyclo[2.2.2]octan-3-one[4-(phenylamino)-6-(1-piperidinyl)-1,3,5-triazin-2-yl]hydrazone, 0.1 microg/ml minimum inhibitory concentration, potent competitive inhibitor of the substrate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | Mycobacterium tuberculosis variant bovis BCG | |
L-tryptophan | tryptophan at high concentrations has definite inhibitory activity against Mycobacterium tuberculosis but does not antagonize the activity of ATB107 | Mycobacterium tuberculosis | |
additional information | inhibitory effect of ATB107 can not be reversed by the addition of tryptophan | Mycobacterium tuberculosis | |
additional information | inhibitory effect of ATB107 can not be reversed by the addition of tryptophan | Mycobacterium tuberculosis H37Ra | |
tryptophan | tryptophan at high concentrations has definite inhibitory activity against Mycobacterium tuberculosis but does not antagonize the activity of ATB107 | Mycobacterium tuberculosis H37Ra |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | increased Km value in the presence of ATB107 | Mycobacterium tuberculosis | |
1.13 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | increased Km value in the presence of ATB107 | Mycobacterium tuberculosis H37Ra | |
1.19 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | H170A site directed mutagenesis | Mycobacterium tuberculosis | |
1.19 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | H170A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
1.42 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L193A site directed mutagenesis | Mycobacterium tuberculosis | |
1.42 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L193A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | S64A site directed mutagenesis | Mycobacterium tuberculosis | |
1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | S64A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | V169A site directed mutagenesis | Mycobacterium tuberculosis | |
1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | V169A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
1.81 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L196A site directed mutagenesis | Mycobacterium tuberculosis | |
1.81 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L196A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
2.49 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | P63A site directed mutagenesis | Mycobacterium tuberculosis | |
2.49 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | P63A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
2.57 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N192A site directed mutagenesis | Mycobacterium tuberculosis | |
2.57 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N192A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
6.63 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | R191A site directed mutagenesis | Mycobacterium tuberculosis | |
6.63 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | R191A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
20.34 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N189A site directed mutagenesis | Mycobacterium tuberculosis | |
20.34 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N189A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
21.67 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | E168A site directed mutagenesis | Mycobacterium tuberculosis | |
21.67 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | E168A site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WFX7 | - |
- |
Mycobacterium tuberculosis H37Ra | A5U2W7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WFX7 | - |
- |
Mycobacterium tuberculosis variant bovis BCG | A1KJ27 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis variant bovis BCG | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis H37Ra | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis H37Rv | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | one typical (beta /alpha)8-barrel structure | Mycobacterium tuberculosis |
More | one typical (beta /alpha)8-barrel structure | Mycobacterium tuberculosis H37Ra |
Synonyms | Comment | Organism |
---|---|---|
IGPS | - |
Mycobacterium tuberculosis |
IGPS | - |
Mycobacterium tuberculosis variant bovis BCG |
IGPS | - |
Mycobacterium tuberculosis H37Ra |
indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis |
indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis variant bovis BCG |
indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis H37Ra |
mIGPS | - |
Mycobacterium tuberculosis |
mIGPS | - |
Mycobacterium tuberculosis variant bovis BCG |
mIGPS | - |
Mycobacterium tuberculosis H37Ra |