Protein Variants | Comment | Organism |
---|---|---|
L5V | Leu5Val without effect on turnover-number and Km | Escherichia coli |
additional information | - |
Escherichia coli |
additional information | mutant enzyme with deletion of 8 terminal residues is soluble and has the same turnover number as the wild-type protein but a 220-fold greater Km-value | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000045 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type enzyme | Saccharolobus solfataricus | |
0.00021 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | mutant Leu5Val | Escherichia coli | |
0.00034 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type enzyme | Escherichia coli | |
0.0099 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | truncated mutant | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Escherichia coli | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? | |
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Saccharolobus solfataricus | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type enzyme | Saccharolobus solfataricus | |
0.041 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | truncated mutant | Saccharolobus solfataricus | |
2 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | mutant Leu5Val | Escherichia coli | |
2.2 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type enzyme | Escherichia coli |