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Literature summary for 4.1.1.46 extracted from
- Biochemical characterization and substrate profiling of a reversible 2,3-dihydroxybenzoic acid decarboxylase for biocatalytic Kolbe-Schmitt reaction (2018), Enzyme Microb. Technol., 113, 37-43 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Fusarium odoratissimum NRRL 54006 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA | Fusarium odoratissimum NRRL 54006 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.2 | - |
2,3-Dihydroxybenzoate | pH 6.0, 40°C | Fusarium odoratissimum NRRL 54006 |  |
| 24.9 | - |
catechol | pH 6.0, 40°C | Fusarium odoratissimum NRRL 54006 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 143600 | - |
gel filtration | Fusarium odoratissimum NRRL 54006 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium odoratissimum NRRL 54006 | X0JT48 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-Dihydroxybenzoate | - |
Fusarium odoratissimum NRRL 54006 | Catechol + CO2 | - |
r | |
| 3-fluoro-2-hydroxybenzoate | - |
Fusarium odoratissimum NRRL 54006 | 2-fluorophenol + CO2 | - |
r | |
| 3-methoxy-6-hydroxybenzoate | - |
Fusarium odoratissimum NRRL 54006 | 3-methoxyphenol + CO2 | - |
r | |
| 3-methoxyphenol + CO2 | - |
Fusarium odoratissimum NRRL 54006 | 3-methoxy-6-hydroxybenzoate | - |
? | |
| catechol + CO2 | - |
Fusarium odoratissimum NRRL 54006 | 2,3-dihydroxybenzoate | - |
r | |
| additional information | enzyme catalyzes the decarboxylation of various substituted salicylic acids with different groups at varied positions except 5-position and the carboxylation of phenol and the substituted phenols | Fusarium odoratissimum NRRL 54006 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 39000, SDS-PAGE | Fusarium odoratissimum NRRL 54006 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FOIG_04675 | - |
Fusarium odoratissimum NRRL 54006 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Fusarium odoratissimum NRRL 54006 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Fusarium odoratissimum NRRL 54006 |
| 50 | - |
2 h, 45% residual decarboxylase activity, 40% residual carboxylase activity | Fusarium odoratissimum NRRL 54006 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.78 | - |
catechol | pH 6.0, 40°C | Fusarium odoratissimum NRRL 54006 | |
| 27.17 | - |
2,3-Dihydroxybenzoate | pH 6.0, 40°C | Fusarium odoratissimum NRRL 54006 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Fusarium odoratissimum NRRL 54006 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
catechol | pH 6.0, 40°C | Fusarium odoratissimum NRRL 54006 | |
| 3.38 | - |
2,3-Dihydroxybenzoate | pH 6.0, 40°C | Fusarium odoratissimum NRRL 54006 | |
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