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Literature summary for 4.1.1.45 extracted from

  • Li, T.; Walker, A.L.; Iwaki, H.; Hasegawa, Y.; Liu, A.
    Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a pentacoordinate mononuclear metallocofactor (2005), J. Am. Chem. Soc., 127, 12282-12290.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0049
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Cd2+ Pseudomonas fluorescens
0.0067
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Mn2+ Pseudomonas fluorescens
0.0232
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Co2+ Pseudomonas fluorescens
0.0921
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Fe2+ Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates Pseudomonas fluorescens
Co2+ activates Pseudomonas fluorescens
Fe2+ activates Pseudomonas fluorescens
Mn2+ activates Pseudomonas fluorescens
additional information Fe3+, Cu2+ not activating Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 SDS-PAGE Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
 25 mM Hepes buffer at pH 7.0 and 25°C, under not saturating conditions. ACSMD loaded with 1 molar equivalent Mn2+ Pseudomonas fluorescens
1.7
-
 25 mM Hepes buffer at pH 7.0 and 25°C, under not saturating conditions. ACSMD loaded with 1 molar equivalent Cd2+ Pseudomonas fluorescens
2.9
-
 25 mM Hepes buffer at pH 7.0 and 25°C, under not saturating conditions. ACSMD loaded with 1 molar equivalent Fe2+ Pseudomonas fluorescens
5.9
-
 25 mM Hepes buffer at pH 7.0 and 25°C, under not saturating conditions. ACSMD loaded with 1 molar equivalent Co2+ Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate
-
 Pseudomonas fluorescens 2-aminomuconate-6-semialdehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ACMSD
-
 Pseudomonas fluorescens
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase
-
 Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Mn2+ Pseudomonas fluorescens
1.7
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Cd2+ Pseudomonas fluorescens
7.3
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Co2+ Pseudomonas fluorescens
10.2
-
 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase?loaded with 1 molar equivalent Fe2+ Pseudomonas fluorescens