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Literature summary for 4.1.1.43 extracted from

  • Asakawa, T.; Wada, H.; Yamano, T.
    Enzymatic conversion of phenylpyruvate to phenylacetate (1968), Biochim. Biophys. Acta, 170, 375-391.
    View publication on PubMed

General Stability

General Stability Organism
thiamine diphosphate stabilizes Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Acinetobacter sp.
PCMB
-
Acinetobacter sp.
Zn2+
-
Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
phenylpyruvate
-
Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
other name: Achromobacter eurydice
-

Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Oxo-gamma-ethylthiobutanoate at approximately 30% of the activity with phenylpyruvate Acinetobacter sp. beta-Ethiopropanal + CO2
-
?
2-Oxo-isohexanoate at approximately 5% of the activity with phenylpyruvate Acinetobacter sp. ?
-
?
2-Oxohexanoate at approximately 31% of the activity with phenylpyruvate Acinetobacter sp. Pentanal + CO2
-
?
4-methylthio-2-oxobutanoate at approximately 23% of the activity with phenylpyruvate Acinetobacter sp. beta-methiopropanal + CO2
-
?
Indolepyruvate at 48% of the activity with phenylpyruvate Acinetobacter sp. Indoleethanal + CO2
-
?
Phenylpyruvate
-
Acinetobacter sp. Phenylacetaldehyde + CO2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
30°C, 1 h, about 60% loss of activity Acinetobacter sp.
6.3 6.4 30°C, 1 h, completely stable, maximal stability Acinetobacter sp.
8.8
-
30°C, 1 h, 80% loss of activity Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate Km: 0.0013-0.0017 mM Acinetobacter sp.