Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.39 extracted from

  • Yoshida, S.; Atomi, H.; Imanaka, T.
    Engineering of a type III Rubisco from a hyperthermophilic archaeon in order to enhance catalytic performance in mesophilic host cells (2007), Appl. Environ. Microbiol., 73, 6254-6261.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.39

Activating Compound

Activating Compound Comment Organism Structure
additional information a solution containing 10 mM NaHCO3, 10 mM MgCl2, and 10 mM Bicine-NaOH (pH 8.3) is used for enzyme activation Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)CodonPlus RIL cells Thermococcus kodakarensis

Protein Variants

Protein Variants Comment Organism
additional information Mutants SP1 and SP2 harbor mutations in the nonconserved residues of loop 6 leading to strongly decreased specific activities. Within alpha-helix 6, SP3 contains a single amino acid substitution from valine to threonine leading to increased specific activity. Mutant SP4 has substitutions in five consecutive amino acid residues leading to increased specific activity. SP5 has a 6-amino-acid substitution in alpha-helix 6 leading to decreased specific activity, while SP6 has 11 substitutions in alpha-helix 6 leading to 31% increased specific activity. In SP7, the entire loop 6/alpha-helix 6 region is replaced with the corresponding region of spinach Rubisco resulting in strongly depressed specific activity. Thermococcus kodakarensis
V330T negative effect on Rbc activity Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 CO2 wild type enzyme, at 25°C Thermococcus kodakarensis
0.9
-
 O2 wild type enzyme, at 25°C Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
PD-10 gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Thermococcus kodakarensis 3-phospho-D-glycerate
-
 ?
D-ribulose 1,5-bisphosphate + O2
-
 Thermococcus kodakarensis 3-phospho-D-glycerate + 2-phosphoglycolate
-
 ?

Synonyms

Synonyms Comment Organism
Rbc
-
 Thermococcus kodakarensis
Rubisco
-
 Thermococcus kodakarensis
type III ribulose 1,5-bisphosphate carboxylase/oxygenase
-
 Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90 100 the half life of the wild type enzyme is about 5fold lower at 100°C than at 90°C Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 CO2 wild type enzyme, at 25°C Thermococcus kodakarensis
0.38
-
 O2 wild type enzyme, at 25°C Thermococcus kodakarensis