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Literature summary for 4.1.1.38 extracted from

  • Lochmueller, H.; Wood, H.G.; Davis, J.J.
    Phosphoenolpyruvate carboxytransphosphorylase. II. Crystallization and properties (1966), J. Biol. Chem., 241, 5678-5691.
    View publication on PubMed
Search for more literature for 4.1.1.38

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Propionibacterium freudenreichii subsp. shermanii

General Stability

General Stability Organism
0.05 M phosphate buffer, 0.01 M or 0.001 M thiol, 0.01 mg protein per ml, stable Propionibacterium freudenreichii subsp. shermanii
30 h dialysis against phosphate buffer containing 1 mM mercaptoethanol, with a change of buffer at 15 h, stable Propionibacterium freudenreichii subsp. shermanii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Propionibacterium freudenreichii subsp. shermanii
diphosphate at 1 mM Propionibacterium freudenreichii subsp. shermanii
DL-malate
-
 Propionibacterium freudenreichii subsp. shermanii
EDTA
-
 Propionibacterium freudenreichii subsp. shermanii
glycylglycine higher inhibition at pH 7.8 than at pH 6.5 Propionibacterium freudenreichii subsp. shermanii
KCl at pH 6.5 Propionibacterium freudenreichii subsp. shermanii
MgCl2 at 7 mM Propionibacterium freudenreichii subsp. shermanii
NaCl at pH 6.5 Propionibacterium freudenreichii subsp. shermanii
oxaloacetate at 7 mM Propionibacterium freudenreichii subsp. shermanii
PCMB
-
 Propionibacterium freudenreichii subsp. shermanii
SO42- competitive against phosphate Propionibacterium freudenreichii subsp. shermanii
Tris-HCl higher inhibition at pH 7.8 than at pH 6.5 Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mn2+ or Mg2+ in activation Propionibacterium freudenreichii subsp. shermanii
Mg2+ Mn2+ or Mg2+ required Propionibacterium freudenreichii subsp. shermanii
Mn2+ Mn2+ or Mg2+ required Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
430000
-
 low speed sedimentation without reaching equilibrium Propionibacterium freudenreichii subsp. shermanii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Propionibacterium freudenreichii subsp. shermanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphate + phosphoenolpyruvate + CO2
-
 Propionibacterium freudenreichii subsp. shermanii diphosphate + oxaloacetate
-
 r
phosphoenolpyruvate + phosphate reaction is catalyzed in absence of CO2 Propionibacterium freudenreichii subsp. shermanii pyruvate + diphosphate
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1 8 formation of oxaloacetate Propionibacterium freudenreichii subsp. shermanii
7 7.5 formation of phosphoenolpyruvate from oxaloacetate and diphosphate Propionibacterium freudenreichii subsp. shermanii

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 35% of maximal activity, pH 8.5: about 55% of maximal activity, formation of oxalacetate Propionibacterium freudenreichii subsp. shermanii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 inactivation, even at 0°C Propionibacterium freudenreichii subsp. shermanii
4.5
-
 25°C, 5 h, little loss of activity Propionibacterium freudenreichii subsp. shermanii
8.2
-
 25°C, 0.2 M NaHCO3, 60% loss of activity after 30 min, rapid loss of activity above pH 8.2 Propionibacterium freudenreichii subsp. shermanii