Crystallization (Comment) | Organism |
---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
General Stability | Organism |
---|---|
0.05 M phosphate buffer, 0.01 M or 0.001 M thiol, 0.01 mg protein per ml, stable | Propionibacterium freudenreichii subsp. shermanii |
30 h dialysis against phosphate buffer containing 1 mM mercaptoethanol, with a change of buffer at 15 h, stable | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Propionibacterium freudenreichii subsp. shermanii | |
diphosphate | at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
DL-malate | - |
Propionibacterium freudenreichii subsp. shermanii | |
EDTA | - |
Propionibacterium freudenreichii subsp. shermanii | |
glycylglycine | higher inhibition at pH 7.8 than at pH 6.5 | Propionibacterium freudenreichii subsp. shermanii | |
KCl | at pH 6.5 | Propionibacterium freudenreichii subsp. shermanii | |
MgCl2 | at 7 mM | Propionibacterium freudenreichii subsp. shermanii | |
NaCl | at pH 6.5 | Propionibacterium freudenreichii subsp. shermanii | |
oxaloacetate | at 7 mM | Propionibacterium freudenreichii subsp. shermanii | |
PCMB | - |
Propionibacterium freudenreichii subsp. shermanii | |
SO42- | competitive against phosphate | Propionibacterium freudenreichii subsp. shermanii | |
Tris-HCl | higher inhibition at pH 7.8 than at pH 6.5 | Propionibacterium freudenreichii subsp. shermanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mn2+ or Mg2+ in activation | Propionibacterium freudenreichii subsp. shermanii | |
Mg2+ | Mn2+ or Mg2+ required | Propionibacterium freudenreichii subsp. shermanii | |
Mn2+ | Mn2+ or Mg2+ required | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
430000 | - |
low speed sedimentation without reaching equilibrium | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphate + phosphoenolpyruvate + CO2 | - |
Propionibacterium freudenreichii subsp. shermanii | diphosphate + oxaloacetate | - |
r | |
phosphoenolpyruvate + phosphate | reaction is catalyzed in absence of CO2 | Propionibacterium freudenreichii subsp. shermanii | pyruvate + diphosphate | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | 8 | formation of oxaloacetate | Propionibacterium freudenreichii subsp. shermanii |
7 | 7.5 | formation of phosphoenolpyruvate from oxaloacetate and diphosphate | Propionibacterium freudenreichii subsp. shermanii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH 5.5: about 35% of maximal activity, pH 8.5: about 55% of maximal activity, formation of oxalacetate | Propionibacterium freudenreichii subsp. shermanii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
inactivation, even at 0°C | Propionibacterium freudenreichii subsp. shermanii |
4.5 | - |
25°C, 5 h, little loss of activity | Propionibacterium freudenreichii subsp. shermanii |
8.2 | - |
25°C, 0.2 M NaHCO3, 60% loss of activity after 30 min, rapid loss of activity above pH 8.2 | Propionibacterium freudenreichii subsp. shermanii |