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Literature summary for 4.1.1.35 extracted from

  • Gustine, D.L.; Hso-Freng Yuan, D.; Kindel, P.K.
    Uridine diphosphate D-glucuronic acid cyclase and uridine diphosphate D-glucuronic acid carboxy-lyase I from Lemna minor. Purification, characterization, and separation from uridine diphosphate D-glucuronic acid carboxy-lyase II (1975), Arch. Biochem. Biophys., 170, 82-91.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
NH4Cl activates Lemna minor

General Stability

General Stability Organism
stable when stored at -90°C and thawed and frozen three times, at days 0, 50 and 100 Lemna minor

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Lemna minor
KCl
-
 Lemna minor
NEM
-
 Lemna minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
 UDPglucuronate
-
 Lemna minor

Organism

Organism UniProt Comment Textmining
Lemna minor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lemna minor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.98
-
-
 Lemna minor

Storage Stability

Storage Stability Organism
-20°C or -90°C, 60% w/v glycerol, stable for at least 100 days Lemna minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucuronate
-
 Lemna minor UDP-xylose + CO2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 rapid inactivation without glycerol Lemna minor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Lemna minor

Cofactor

Cofactor Comment Organism Structure
NAD+ required Lemna minor
NAD+ optimal concentration: 1-2 mM Lemna minor