Application | Comment | Organism |
---|---|---|
biofuel production | enzyme establishes a possible route for biological production of petroleum based fuels and plastics by producing isobutene enzymatically | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells. Whole cells of Eschercihia coli produced ScMDD with an N-terminal 6xHis tag form isobutene from 3-hydroxy-3-methylbutyrate at a rate 0.154 nmol/h * g cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
R74H | mutant increases specific activity on non-native substrate (3-hydroxy-3-methylbutyrate) by 4.8fold | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
44120 | - |
calculated from protein sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-diphosphomevalonate | Saccharomyces cerevisiae | - |
ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32377 | - |
- |
Purification (Comment) | Organism |
---|---|
both, the wild-type 6XHis tag ScMDD and the most active mutant R74H ScMDD2 purified by nickel affinity chromatography under anaerobic conditions, 90 to 95% homogenous | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
improved mutants ScMDD1 (I145F) and ScMDD2 (R74H) produce 3 and 5.888 nmol/h * g cells, respectively, which are 19- and 38fold increases compared to the wild type 6xHis ScMDD | Saccharomyces cerevisiae |
0.00000133 | - |
wild-type, 6xHis tagged, produced in Escherichia coli, 37°C, pH 7.4 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxy-3-methylbutyrate | - |
Saccharomyces cerevisiae | ADP + isobutene + CO2 | isobutene is identified by gas chromatography with flame ionization detection as well as by GC-mass spectrometry. Isobutene is an important commercial chemical used for the synthesis of butyl rubber, terephthalic acid, specialty chemicals, and a gasoline performance additive known as alkylate | ? | |
ATP + 5-diphosphomevalonate | - |
Saccharomyces cerevisiae | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diphosphomevalonate decarboxylase | - |
Saccharomyces cerevisiae |
ScMDD | - |
Saccharomyces cerevisiae |
ScMDD1 | improved mutant I145F | Saccharomyces cerevisiae |
ScMDD2 | improved mutant R74H | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |