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Literature summary for 4.1.1.33 extracted from

  • Krepkiy, D.V.; Miziorko, H.M.
    Investigation of the functional contributions of invariant serine residues in yeast mevalonate diphosphate decarboxylase (2005), Biochemistry, 44, 2671-2677.
    View publication on PubMed
Search for more literature for 4.1.1.33

Protein Variants

Protein Variants Comment Organism
S120A 8fold diminution in kcat-value Saccharomyces cerevisiae
S121A 42000fold diminution in kcat-value Saccharomyces cerevisiae
S153A 18fold inflation in Kd-value for Mg-ATP, 35fold inflation in Km-value for Mg-ATP Saccharomyces cerevisiae
S155A 26fold inflation in Km-value for Mg-ATP, 16fold inflation in Km-value for diphosphomevalonate Saccharomyces cerevisiae
S36A limited solubility, impaired binding of fluorescent ATP analogue trinitrophenyl-ATP Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
diphosphoglycolylproline
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 ATP wild-type, pH 7.0, 30°C Saccharomyces cerevisiae
0.061
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae
0.065
-
 ATP mutant S36A, pH 7.0, 30°C Saccharomyces cerevisiae
0.065
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae
0.095
-
 5-diphosphomevalonate mutant S121A, pH 7.0, 30°C Saccharomyces cerevisiae
0.123
-
 5-diphosphomevalonate wild-type, pH 7.0, 30°C Saccharomyces cerevisiae
0.267
-
 5-diphosphomevalonate mutant S120A, pH 7.0, 30°C Saccharomyces cerevisiae
0.307
-
 5-diphosphomevalonate mutant S36A, pH 7.0, 30°C Saccharomyces cerevisiae
0.525
-
 ATP mutant S120A, pH 7.0, 30°C Saccharomyces cerevisiae
0.525
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae
0.944
-
 5-diphosphomevalonate mutant S153A, pH 7.0, 30°C Saccharomyces cerevisiae
1.592
-
 ATP mutant S155A, pH 7.0, 30°C Saccharomyces cerevisiae
1.592
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae
1.921
-
 5-diphosphomevalonate mutant S155A, pH 7.0, 30°C Saccharomyces cerevisiae
2.172
-
 ATP mutant S153A, pH 7.0, 30°C Saccharomyces cerevisiae
2.172
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32377
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00015
-
 mutant S121A, pH 7.0, 30°C Saccharomyces cerevisiae
0.8
-
 mutant S120A, pH 7.0, 30°C Saccharomyces cerevisiae
0.85
-
 mutant S155A, pH 7.0, 30°C Saccharomyces cerevisiae
2.19
-
 mutant S153A, pH 7.0, 30°C Saccharomyces cerevisiae
4.36
-
 mutant S36A, pH 7.0, 30°C Saccharomyces cerevisiae
6.4
-
 wild-type, pH 7.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-diphosphomevalonate
-
 Saccharomyces cerevisiae ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?
ATP + 5-diphosphomevalonate
-
 Saccharomyces cerevisiae ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000115
-
 5-diphosphomevalonate mutant S121A, pH 7.0, 30°C Saccharomyces cerevisiae
0.61
-
 5-diphosphomevalonate mutant S120A, pH 7.0, 30°C Saccharomyces cerevisiae
0.65
-
 5-diphosphomevalonate mutant S155A, pH 7.0, 30°C Saccharomyces cerevisiae
1.68
-
 5-diphosphomevalonate mutant S153A, pH 7.0, 30°C Saccharomyces cerevisiae
3.35
-
 5-diphosphomevalonate mutant S36A, pH 7.0, 30°C Saccharomyces cerevisiae
4.9
-
 5-diphosphomevalonate wild-type, pH 7.0, 30°C Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
 diphosphoglycolylproline mutant S120A, pH 7.0, 30°C Saccharomyces cerevisiae
0.066
-
 diphosphoglycolylproline mutant S36A, pH 7.0, 30°C Saccharomyces cerevisiae
0.105
-
 diphosphoglycolylproline wild-type, pH 7.0, 30°C Saccharomyces cerevisiae
1.429
-
 diphosphoglycolylproline mutant S155A, pH 7.0, 30°C Saccharomyces cerevisiae
1.576
-
 diphosphoglycolylproline mutant S153A, pH 7.0, 30°C Saccharomyces cerevisiae