KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-diphosphomevalonate | 37000 MW enzyme form | Rattus norvegicus | |
0.0227 | - |
5-diphosphomevalonate | 45000 MW enzyme form | Rattus norvegicus | |
0.8 | - |
ATP | 37000 MW enzyme form | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Rattus norvegicus | |
Co2+ | Mn2+ and Co2+ are the best activators for the 45000 MW enzyme form | Rattus norvegicus | |
Mg2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Rattus norvegicus | |
Mn2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Rattus norvegicus | |
Mn2+ | Mn2+ is the best activator for the 37000 MW enzyme form | Rattus norvegicus | |
Mn2+ | Mn2+ and Co2+ are the best activators for the 45000 MW enzyme form | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE | Rattus norvegicus |
45000 | - |
2 * 45000, SDS-PAGE | Rattus norvegicus |
90000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | intracellular glycosylation does not contribute to the difference between the 45000 MW form and the 37000 MW form of the enzyme | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
2 enzyme forms: MW 37000 and MW 45000. The MW 37000 enzyme form is induced by the diet containing cholestyramine and pravastin | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | from animals fed a diet containing cholestyramine and pravastin | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-diphosphomevalonate | - |
Rattus norvegicus | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
37000 MW form and 45000 MW form | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | about 35% of maximal activity at pH 4 and at pH 9, 37000 MW enzyme form | Rattus norvegicus |
6 | 9 | pH 6: about 35% of maximal activity, pH 9: about 30% of maximal activity, 45000 MW enzyme form | Rattus norvegicus |