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Literature summary for 4.1.1.33 extracted from
- Purification and characterization of two mevalonate pyrophosphate decarboxylases from rat liver: a novel molecular species of 37 kDa (1997), J. Biochem., 122, 647-654.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
5-diphosphomevalonate | 37000 MW enzyme form | Rattus norvegicus |  |
| 0.0227 | - |
5-diphosphomevalonate | 45000 MW enzyme form | Rattus norvegicus | |
| 0.8 | - |
ATP | 37000 MW enzyme form | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Rattus norvegicus | |
| Co2+ | Mn2+ and Co2+ are the best activators for the 45000 MW enzyme form | Rattus norvegicus | |
| Mg2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Rattus norvegicus | |
| Mn2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Rattus norvegicus | |
| Mn2+ | Mn2+ is the best activator for the 37000 MW enzyme form | Rattus norvegicus | |
| Mn2+ | Mn2+ and Co2+ are the best activators for the 45000 MW enzyme form | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE | Rattus norvegicus |
| 45000 | - |
2 * 45000, SDS-PAGE | Rattus norvegicus |
| 90000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | intracellular glycosylation does not contribute to the difference between the 45000 MW form and the 37000 MW form of the enzyme | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 enzyme forms: MW 37000 and MW 45000. The MW 37000 enzyme form is induced by the diet containing cholestyramine and pravastin | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | from animals fed a diet containing cholestyramine and pravastin | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-diphosphomevalonate | - |
Rattus norvegicus | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 37000, enzyme form is induced by the diet containing cholestyramine and pravastin, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
37000 MW form and 45000 MW form | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | about 35% of maximal activity at pH 4 and at pH 9, 37000 MW enzyme form | Rattus norvegicus |
| 6 | 9 | pH 6: about 35% of maximal activity, pH 9: about 30% of maximal activity, 45000 MW enzyme form | Rattus norvegicus |
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