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Literature summary for 4.1.1.32 extracted from

  • Das, B.; Tandon, V.; Saxena, J.K.; Joshi, S.; Singh, A.R.
    Purification and characterization of phosphoenolpyruvate carboxykinase from Raillietina echinobothrida, a cestode parasite of the domestic fowl (2013), Parasitology, 140, 136-146.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GMP competitive inhibitor Raillietina echinobothrida
GTP competitive inhibitor Raillietina echinobothrida
IMP competitive inhibitor Raillietina echinobothrida
ITP competitive inhibitor Raillietina echinobothrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02412
-
phosphoenolpyruvate with IDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
0.02516
-
GDP in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
0.04252
-
phosphoenolpyruvate with GDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
0.1273
-
IDP in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
4.76
-
CO2 in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required, 4 mM used in assay conditions Raillietina echinobothrida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
high pressure liquid chromatography Raillietina echinobothrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + phosphoenolpyruvate + CO2 Raillietina echinobothrida highest activity GTP + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Raillietina echinobothrida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Blue Sepharose CL 6B affinity chromatography Raillietina echinobothrida

Storage Stability

Storage Stability Organism
-80°C, more than 1 year, the enzyme remains stable Raillietina echinobothrida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + phosphoenolpyruvate + CO2 highest activity Raillietina echinobothrida GTP + oxaloacetate
-
?
IDP + phosphoenolpyruvate + CO2 lower activity compared to GDP Raillietina echinobothrida ITP + oxaloacetate
-
?
additional information no enzyme activity observed when ADP or CDP is used Raillietina echinobothrida ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Raillietina echinobothrida

Synonyms

Synonyms Comment Organism
PEP carboxykinase
-
Raillietina echinobothrida
PEPCK
-
Raillietina echinobothrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Raillietina echinobothrida

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8 there is a steep rise in the enzyme activity (about 2fold) between the pH 6.6 and pH 7.4, although a sharp decline is observed within a narrow range (pH 7.4–8.0) Raillietina echinobothrida

Cofactor

Cofactor Comment Organism Structure
GDP
-
Raillietina echinobothrida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.07642
-
GTP in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
0.1864
-
GMP in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
0.2264
-
ITP in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida
0.3483
-
IMP in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication Raillietina echinobothrida