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Literature summary for 4.1.1.32 extracted from
- Phosphoenolpyruvate carboxykinase from ribbed mussel gill tissue: reactivity with metal ions, kinetics, and action of 3-mercaptopicolinic acid (1991), J. Exp. Zool., 257, 285-298.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Aminopicolinic acid | - |
Modiolus demissus |  |
| 3-Mercaptopicolinic acid | strong | Modiolus demissus | |
| ADP | uncompetitive with respect to IDP, partially competitive with respect to phosphoenolpyruvate | Modiolus demissus | |
| ATP | strong | Modiolus demissus | |
| Hydroxymalonate | slight | Modiolus demissus | |
| KCl | 120 mM, 50% inhibition | Modiolus demissus | |
| NaCl | 115 mM, 50% inhibition | Modiolus demissus | |
| Zn2+ | activates at low levels, inhibits above 0.3 mM | Modiolus demissus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
GDP | in presence of 1 mM MnCl2 and 1 mM MgCl2 | Modiolus demissus | |
| 0.024 | - |
oxaloacetate | in presence of 1 mM MnCl2 and 1 mM MgCl2 | Modiolus demissus | |
| 0.046 | - |
IDP | in presence of 1 mM MnCl2 and 1 mM MgCl2 | Modiolus demissus | |
| 0.055 | - |
GTP | in presence of 1 mM MnCl2 and 1 mM MgCl2 | Modiolus demissus | |
| 0.396 | - |
phosphoenolpyruvate | in presence of 1 mM MnCl2 and 1 mM MgCl2 | Modiolus demissus | |
| 13 | - |
CO2 | in presence of 1 mM MnCl2 and 1 mM MgCl2 | Modiolus demissus | |
| 13 | - |
CO2 | CO2 in form of HCO3- | Modiolus demissus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Modiolus demissus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mn2+ or Co2+ required | Modiolus demissus | |
| Mn2+ | Km: 0.28 mM | Modiolus demissus | |
| Mn2+ | Mn2+ or Co2+ required | Modiolus demissus | |
| Zn2+ | activates at low levels, inhibits above 0.3 mM | Modiolus demissus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Modiolus demissus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Modiolus demissus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.81 | - |
- |
Modiolus demissus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP + phosphoenolpyruvate + CO2 | - |
Modiolus demissus | GTP + oxaloacetate | - |
? | |
| GTP + oxaloacetate | - |
Modiolus demissus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
| IDP + phosphoenolpyruvate + CO2 | - |
Modiolus demissus | ITP + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Modiolus demissus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Modiolus demissus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 7.5 | about 50% of maximal activity at pH 6.3 and pH 7.5 | Modiolus demissus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 115 | - |
NaCl | - |
Modiolus demissus | |
| 120 | - |
KCl | - |
Modiolus demissus | |
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