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Literature summary for 4.1.1.32 extracted from
- Purification and characteriation of phosphoenolpyruvate carboxykinase from the parasitic helminth Ascaris suum (1986), J. Biol. Chem., 261, 13049-13055.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | competitive against GTP | Ascaris suum |  |
| ITP | competitive against GTP, IDP and phosphoenolpyruvate | Ascaris suum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
GDP | - |
Ascaris suum | |
| 0.12 | - |
phosphoenolpyruvate | - |
Ascaris suum | |
| 0.182 | - |
IDP | - |
Ascaris suum | |
| 6.7 | - |
CO2 | with IDP as cosubstrate, Mn2+ does not affect the Km | Ascaris suum | |
| 6.7 | - |
CO2 | CO2 in form of HCO3- | Ascaris suum | |
| 7.1 | - |
CO2 | with GDP as cosubstrate, Mn2+ does not affect the Km | Ascaris suum | |
| 7.1 | - |
CO2 | CO2 in form of HCO3- | Ascaris suum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Ascaris suum | |
| Co2+ | maximal activity at 4 mM | Ascaris suum | |
| Mg2+ | no activation | Ascaris suum | |
| Mn2+ | activates | Ascaris suum | |
| Mn2+ | Km: 0.77 mM | Ascaris suum | |
| Mn2+ | binds directly to the enzyme and binds to the nucleotide resulting in the metal-nucleotide complex | Ascaris suum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
gel filtration | Ascaris suum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ascaris suum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.93 | - |
- |
Ascaris suum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 0.05 M potassium phosphate, pH 6.8, 0.2 M sucrose, stable for at least 21 months | Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP + phosphoenolpyruvate + CO2 | - |
Ascaris suum | GTP + oxaloacetate | - |
? | |
| IDP + phosphoenolpyruvate + CO2 | - |
Ascaris suum | ITP + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 75000-80000, SDS-PAGE | Ascaris suum |
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