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Literature summary for 4.1.1.32 extracted from
- Purification and molecular characterization of mitochondrial phosphoenolpyruvate carboxykinase from bullfrog (Rana catesbeiana) liver (1980), J. Biochem., 88, 1239-1249.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GMP | competitive with GDP | Lithobates catesbeianus |  |
| iodoacetamide | guanine nucleotides, IDP and Mn2+ protect | Lithobates catesbeianus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Lithobates catesbeianus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | absolutely required for phosphoenolpyruvate carboxylation | Lithobates catesbeianus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Lithobates catesbeianus |
| 70000 | - |
1 * 70000, SDS-PAGE | Lithobates catesbeianus |
| 71000 | - |
glycerol density gradient centrifugation | Lithobates catesbeianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lithobates catesbeianus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Lithobates catesbeianus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.7 | - |
- |
Lithobates catesbeianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + oxaloacetate | - |
Lithobates catesbeianus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
| additional information | ADP is completely inactive in carboxylation of oxaloacetate | Lithobates catesbeianus | ? | - |
? | |
| UDP + phosphoenolpyruvate + CO2 | very low activity | Lithobates catesbeianus | UTP + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Lithobates catesbeianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
phosphoenolpyruvate carboxylation and exchange reaction in presence of Mn2+ | Lithobates catesbeianus |
| 7.5 | - |
oxaloacetate decarboxylation in presence of Mn2+ | Lithobates catesbeianus |
| 7.5 | - |
oxaloacetate decarboxylation and exchange reaction in presence of Mg2+ | Lithobates catesbeianus |
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Release 2023.1 (January 2023)
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