Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.31 extracted from

  • Takeya, M.; Hirai, M.; Osanai, T.
    Allosteric inhibition of phosphoenolpyruvate carboxylases is determined by a single amino acid residue in cyanobacteria (2017), Sci. Rep., 7, 41080 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.31

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA 1 mM, 111% of initial activity Synechocystis sp. PCC 6803
succinate 1 mM, 124% of initial activity Synechocystis sp. PCC 6803

Protein Variants

Protein Variants Comment Organism
E946K mutation renders the enzyme sensitive to inhibition by malate, aspartate, and fumarate Nostoc sp. PCC 7120 = FACHB-418
E954K mutation renders the enzyme sensitive to inhibition by malate, aspartate, and fumarate Synechocystis sp. PCC 6803
S967V loss of activity Synechocystis sp. PCC 6803

Inhibitors

Inhibitors Comment Organism Structure
citrate 1 mM, 86% residual activity Synechocystis sp. PCC 6803
fumarate 1 mM, 75% residual activity Synechocystis sp. PCC 6803
L-aspartate 1 mM, 85% residual activity for wild-type, 60% for mutant K954E, respectively Synechocystis sp. PCC 6803
L-malate 1 mM, 30% residual activity, 80% for mutant K946E, respectively Nostoc sp. PCC 7120 = FACHB-418
L-malate 1 mM, 77% residual activity, 60% for mutant K954E, respectively Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 phosphoenolpyruvate wild-type, pH 8.0, 35°C Nostoc sp. PCC 7120 = FACHB-418
0.24
-
 HCO3- wild-type, pH 8.0, 35°C Nostoc sp. PCC 7120 = FACHB-418
0.25
-
 HCO3- mutant K946E, pH 8.0, 35°C Nostoc sp. PCC 7120 = FACHB-418
0.34
-
 phosphoenolpyruvate wild-type, pH 7.3, 30°C Synechocystis sp. PCC 6803
0.76
-
 HCO3- mutant K954E, pH 7.3, 30°C Synechocystis sp. PCC 6803
0.8
-
 HCO3- wild-type, pH 7.3, 30°C Synechocystis sp. PCC 6803
0.8
-
 phosphoenolpyruvate mutant K946E, pH 8.0, 35°C Nostoc sp. PCC 7120 = FACHB-418
0.82
-
 phosphoenolpyruvate mutant K954E, pH 7.3, 30°C Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 P28594
-
-
Synechocystis sp. PCC 6803 P74299
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + HCO3-
-
 Synechocystis sp. PCC 6803 phosphate + oxaloacetate
-
 ?
phosphoenolpyruvate + HCO3-
-
 Nostoc sp. PCC 7120 = FACHB-418 phosphate + oxaloacetate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Synechocystis sp. PCC 6803
35
-
-
 Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Synechocystis sp. PCC 6803
8
-
-
 Nostoc sp. PCC 7120 = FACHB-418