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Literature summary for 4.1.1.31 extracted from

  • Sako, Y.; Takai, K.; Nishizaka, T.; Ishida, Y.
    Biochemical relationship of phosphoenolpyruvate carboxylases (PEPCs) from thermophilic archaea (1997), FEMS Microbiol. Lett., 153, 159-165.
No PubMed abstract available
Search for more literature for 4.1.1.31

Inhibitors

Inhibitors Comment Organism Structure
L-aspartate 4 mM, complete inhibition Sulfolobus acidocaldarius
L-malate 10 mM, 48% inhibition Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme absolutely requires Mg2+ and cannot utilize Mn2+ instead, maximal activity at 1 mM, decrease of activity at higher concentrations Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 4 * 60000, SDS-PAGE Sulfolobus acidocaldarius
260000
-
 gel filtration Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius DSM 639
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.77
-
 pH 8.0, 80°C Sulfolobus acidocaldarius

Subunits

Subunits Comment Organism
homotetramer 4 * 60000, SDS-PAGE Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 70°C: about 60% of maximal activity, 95°C: about 55% of maximal activity Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 2 h, no loss of activity Sulfolobus acidocaldarius
90
-
 60 min, 50% loss of activity Sulfolobus acidocaldarius
95
-
 10 min, 50% loss of activity Sulfolobus acidocaldarius
100
-
 1 min, 50% loss of activity Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 Tris-H2SO4 buffer Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 65% of maximal activity, pH 9.5: about 70% of maximal activity, Tris-H2SO4 buffer Sulfolobus acidocaldarius