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Literature summary for 4.1.1.31 extracted from

  • Xu, W.; Ahmed, S.; Moriyama, H.; Chollet, R.
    The importance of the strictly conserved, C-terminal glycine residue in phosphoenolpyruvate carboxylase for overall catalysis: mutagenesis and truncation of GLY-961 in the sorghum C4 leaf isoform (2006), J. Biol. Chem., 281, 17238-17245.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned as a Histag-fusion protein in an Escherichia coli PEPC- (Ppc-) strain Sorghum bicolor

Protein Variants

Protein Variants Comment Organism
delataC1 deletion of the last c-terminal amino acid. kcat: 9.5/sec (phosphoenolpyruvate),12.6% of wild type catalytic activity Sorghum bicolor
delataC4 deletion of the last 4 c-terminal amino acids. kcat: 0.174/sec (phosphoenolpyruvate), 0.14% wild type catalytic activity Sorghum bicolor
G961A kcat: 17.4/sec (phosphoenolpyruvate), 24.3% of wild type catalytic activity Sorghum bicolor
G961V kcat: 7.2/sec (phosphoenolpyruvate), 8.5% of wild type catalytic activity Sorghum bicolor
additional information it is shown that even a modest, neutral alteration of the PEPC C-terminal hydrogen atom side chain is detrimental to enzyme function Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
SDS-PAGE Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor P15804 sorghum C4 isoform
-

Purification (Commentary)

Purification (Comment) Organism
affinity purification Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Sorghum bicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the four C-terminal mutant enzymes display varying degrees of PEPC activity in vitro ranging from 23% of wild-type with the modest G961A substitution to only 0.2% for the DELTAC4-truncated form Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Sorghum bicolor phosphate + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
PEPC
-
Sorghum bicolor
Phosphoenolpyruvate carboxylase
-
Sorghum bicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sorghum bicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.174
-
phosphoenolpyruvate DELTAC4, deletion of the last 4 c-terminal amino acids Sorghum bicolor
7.2
-
phosphoenolpyruvate G961V Sorghum bicolor
9.5
-
phosphoenolpyruvate DELTAC1, deletion of the last c-terminal amino acid Sorghum bicolor
17.4
-
phosphoenolpyruvate G961A Sorghum bicolor
66
-
phosphoenolpyruvate
-
Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Sorghum bicolor