Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 6-phosphate | 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate | Ricinus communis | |
D-glucose 1-phosphate | 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate | Ricinus communis | |
D-glucose 6-phosphate | 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate | Ricinus communis | |
D-glucose 6-phosphate | 2 mM, 2fold increase of activity of PEPC1 at pH 7.3, 1.18fold increase in activity of PEPC1 at pH 8 | Ricinus communis | |
fructose 6-phosphate | 2 mM, 2fold increase of activity of PEPC1 at pH 7.3, 1.27fold increase in activity of PEPC1 at pH 8 | Ricinus communis | |
glucose 1-phosphate | 2 mM, 1.64fold increase of activity of PEPC1 at pH 7.3, 1.07fold increase in activity of PEPC1 at pH 8 | Ricinus communis | |
glycerol 3-phosphate | 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate | Ricinus communis | |
glycerol 3-phosphate | 2 mM, 1.83fold increase of activity of PEPC1 at pH 7.3, 1.16fold increase in activity of PEPC1 at pH 8 | Ricinus communis | |
malate | 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 2 mM, 65% decreases of activity of PEPC1 at pH 7.3, 13% decrease in activity of PEPC2 at pH 8 | Ricinus communis | |
L-Asp | 2 mM, 95% decreases of activity of PEPC1 at pH 7.3, 49% decrease in activity of PEPC2 at pH 8 | Ricinus communis | |
L-Glu | 2 mM, 96% decreases of activity of PEPC1 at pH 7.3, 22% decrease in activity of PEPC2 at pH 8 | Ricinus communis | |
malate | 2 mM, 96% decreases of activity of PEPC1 at pH 7.3, 59% decrease in activity of PEPC1 at pH 8 | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
phosphoenolpyruvate | pH 8, PEPC1 | Ricinus communis | |
0.06 | - |
phosphoenolpyruvate | pH 8, PEPC2 | Ricinus communis | |
0.12 | - |
phosphoenolpyruvate | pH 7.3, PEPC1 | Ricinus communis | |
0.12 | - |
phosphoenolpyruvate | pH 7.3, PEPC2 | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE | Ricinus communis |
107000 | - |
4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE | Ricinus communis |
107000 | - |
4 * 107000, PEPC1, SDS-PAGE | Ricinus communis |
410000 | - |
gel filtration, PEPC1 | Ricinus communis |
681000 | - |
gel filtration, PEPC2 | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
L. Cv. Baker 296 | - |
Purification (Comment) | Organism |
---|---|
- |
Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.2 | - |
PEPC1 | Ricinus communis |
29.2 | - |
PEPC2 | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Ricinus communis | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE | Ricinus communis |
tetramer | 4 * 107000, PEPC1, SDS-PAGE | Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
PEPC1 | - |
Ricinus communis |
PEPC2 | - |
Ricinus communis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
3 min, no loss of activity, PEPC1 | Ricinus communis |
35 | - |
3 min, 19% loss of activity, PEPC1 | Ricinus communis |
40 | - |
3 min, 25% loss of activity, PEPC1 | Ricinus communis |
45 | - |
3 min, 40% loss of activity, PEPC1 | Ricinus communis |
45 | - |
3 min, no loss of activity, PEPC2 | Ricinus communis |
50 | - |
3 min, 20% loss of activity, PEPC2 | Ricinus communis |
50 | - |
3 min, 82% loss of activity, PEPC1 | Ricinus communis |
55 | - |
3 min, complete loss of activity, PEPC1 | Ricinus communis |
55 | - |
3 min, complete loss of activity, PEPC2 | Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
PEPC2 | Ricinus communis |
8.5 | - |
PEPC1 | Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 35% of maximal activity, pH 9.5: about 75% of maximal activity, PEPC2 | Ricinus communis |
7 | 9.6 | pH 7.0: about 60% of maximal activity, pH 9.5: about 90% of maximal activity, PEPC1 | Ricinus communis |