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Literature summary for 4.1.1.31 extracted from

  • Blonde, J.D.; Plaxton, W.C.
    Structural and kinetic properties of high and low molecular mass phosphoenolpyruvate carboxylase isoforms from the endosperm of developing castor oilseeds (2003), J. Biol. Chem., 278, 11867-11873.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 6-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
D-glucose 1-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
D-glucose 6-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
D-glucose 6-phosphate 2 mM, 2fold increase of activity of PEPC1 at pH 7.3, 1.18fold increase in activity of PEPC1 at pH 8 Ricinus communis
fructose 6-phosphate 2 mM, 2fold increase of activity of PEPC1 at pH 7.3, 1.27fold increase in activity of PEPC1 at pH 8 Ricinus communis
glucose 1-phosphate 2 mM, 1.64fold increase of activity of PEPC1 at pH 7.3, 1.07fold increase in activity of PEPC1 at pH 8 Ricinus communis
glycerol 3-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
glycerol 3-phosphate 2 mM, 1.83fold increase of activity of PEPC1 at pH 7.3, 1.16fold increase in activity of PEPC1 at pH 8 Ricinus communis
malate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
ATP 2 mM, 65% decreases of activity of PEPC1 at pH 7.3, 13% decrease in activity of PEPC2 at pH 8 Ricinus communis
L-Asp 2 mM, 95% decreases of activity of PEPC1 at pH 7.3, 49% decrease in activity of PEPC2 at pH 8 Ricinus communis
L-Glu 2 mM, 96% decreases of activity of PEPC1 at pH 7.3, 22% decrease in activity of PEPC2 at pH 8 Ricinus communis
malate 2 mM, 96% decreases of activity of PEPC1 at pH 7.3, 59% decrease in activity of PEPC1 at pH 8 Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
phosphoenolpyruvate pH 8, PEPC1 Ricinus communis
0.06
-
phosphoenolpyruvate pH 8, PEPC2 Ricinus communis
0.12
-
phosphoenolpyruvate pH 7.3, PEPC1 Ricinus communis
0.12
-
phosphoenolpyruvate pH 7.3, PEPC2 Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE Ricinus communis
107000
-
4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE Ricinus communis
107000
-
4 * 107000, PEPC1, SDS-PAGE Ricinus communis
410000
-
gel filtration, PEPC1 Ricinus communis
681000
-
gel filtration, PEPC2 Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
L. Cv. Baker 296
-

Purification (Commentary)

Purification (Comment) Organism
-
Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.2
-
PEPC1 Ricinus communis
29.2
-
PEPC2 Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Ricinus communis phosphate + oxaloacetate
-
?

Subunits

Subunits Comment Organism
octamer 4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE Ricinus communis
tetramer 4 * 107000, PEPC1, SDS-PAGE Ricinus communis

Synonyms

Synonyms Comment Organism
PEPC1
-
Ricinus communis
PEPC2
-
Ricinus communis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
3 min, no loss of activity, PEPC1 Ricinus communis
35
-
3 min, 19% loss of activity, PEPC1 Ricinus communis
40
-
3 min, 25% loss of activity, PEPC1 Ricinus communis
45
-
3 min, 40% loss of activity, PEPC1 Ricinus communis
45
-
3 min, no loss of activity, PEPC2 Ricinus communis
50
-
3 min, 20% loss of activity, PEPC2 Ricinus communis
50
-
3 min, 82% loss of activity, PEPC1 Ricinus communis
55
-
3 min, complete loss of activity, PEPC1 Ricinus communis
55
-
3 min, complete loss of activity, PEPC2 Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
PEPC2 Ricinus communis
8.5
-
PEPC1 Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 35% of maximal activity, pH 9.5: about 75% of maximal activity, PEPC2 Ricinus communis
7 9.6 pH 7.0: about 60% of maximal activity, pH 9.5: about 90% of maximal activity, PEPC1 Ricinus communis