Activating Compound | Comment | Organism | Structure |
---|---|---|---|
propionyl-CoA | absolute requirement for acetyl-CoA or propionyl-CoA | Acetobacter aceti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Acetobacter aceti | |
Asp | - |
Acetobacter aceti | |
citrate | - |
Acetobacter aceti | |
Co2+ | maximal activity at 1 mM Co2+, strong decrease of activity above 4 mM | Acetobacter aceti | |
fumarate | - |
Acetobacter aceti | |
Glu | - |
Acetobacter aceti | |
Mn2+ | maximal activity at 0.5 mM Mn2+, strong decrease of activity above 2 mM | Acetobacter aceti | |
p-hydroxymercuribenzoate | glutathione protects | Acetobacter aceti | |
PCMB | glutathione protects | Acetobacter aceti | |
succinate | - |
Acetobacter aceti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
phosphoenolpyruvate | - |
Acetobacter aceti | |
3 | - |
HCO3- | - |
Acetobacter aceti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, Mn2+, Co2+ and Mg2+. Co2+ is less effective than Mg2+ or Mn2+ | Acetobacter aceti | |
Co2+ | maximal activity at 1 mM Co2+, strong decrease of activity above 4 mM | Acetobacter aceti | |
Co2+ | Km: 0.26 mM | Acetobacter aceti | |
Mg2+ | Km: 0.77 mM | Acetobacter aceti | |
Mg2+ | divalent cation required, Mn2+, Co2+ and Mg2+ | Acetobacter aceti | |
Mn2+ | divalent cation required, Mn2+, Co2+ and Mg2+ | Acetobacter aceti | |
Mn2+ | Km: 0.12 mM | Acetobacter aceti | |
Mn2+ | maximal activity at 0.5 mM Mn2+, strong decrease of activity above 2 mM | Acetobacter aceti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
380000 | 400000 | gel filtration | Acetobacter aceti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter aceti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetobacter aceti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.9 | - |
- |
Acetobacter aceti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Acetobacter aceti | phosphate + oxaloacetate | - |
? | |
phosphoenolpyruvate + HCO3- | - |
Acetobacter aceti | phosphate + oxaloacetate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Acetobacter aceti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | rapid inactivation | Acetobacter aceti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acetobacter aceti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | about 80% of maximal activity at pH 6.5 and at pH 9.0 | Acetobacter aceti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | absolute requirement for acetyl-CoA or propionyl-CoA | Acetobacter aceti |