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Literature summary for 4.1.1.31 extracted from
- Corn leaf phosphoenolpyruvate carboxylases. Purification and properties of two isoenzymes (1977), Arch. Biochem. Biophys., 182, 343-351.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
phosphoenolpyruvate | isoenzyme PC-II | Zea mays |  |
| 0.1 | - |
phosphoenolpyruvate | isoenzyme PC-I | Zea mays | |
| 1.28 | - |
HCO3- | isoenzyme PC-II | Zea mays | |
| 1.45 | - |
HCO3- | isoenzyme PC-I | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Zea mays | |
| Mg2+ | Km for isoenzyme PC-II: 0.015 mM | Zea mays | |
| Mg2+ | Km for isoenzyme PC-I: 0.032 mM | Zea mays | |
| Mn2+ | Km for isoenzyme PC-II: 0.0067 mM | Zea mays | |
| Mn2+ | Km for isoenzyme PC-I: 0.0102 mM | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 225700 | - |
sucrose density gradient centrifugation, isoenzyme PC-I | Zea mays |
| 270800 | - |
sucrose density gradient centrifugation, isoenzyme PC-II | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme PC-I and PC-II | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Zea mays | phosphate + oxaloacetate | - |
? | |
| phosphoenolpyruvate + HCO3- | - |
Zea mays | phosphate + oxaloacetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isoenzyme PC-I | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
50% inactivation after 87 s, isoenzyme PC-I. 50% inactivation after 100 s, isoenzyme PC-II | Zea mays |
| 50 | - |
50% inactivation after 18 s, isoenzyme PC-I. 50% inactivation after 28 s, isoenzyme PC-II | Zea mays |
| 55 | - |
50% inactivation after 13 s, isoenzyme PC-I. 50% inactivation after 17 s, isoenzyme PC-II | Zea mays |
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