BRENDA - Enzyme Database
show all sequences of 4.1.1.30

Pantothenylcysteine, a precursor of pantetheine in Lactobacillus helveticus

Brown, G.M.; J. Biol. Chem. 226, 651-661 (1957)

Data extracted from this reference:

General Stability
General Stability
Organism
dialysed at 4°C, pH 5.5-7.5, without loss in activity, cell extract
Lactobacillus delbrueckii subsp. bulgaricus
dialysed at 4°C, pH 5.5-7.5, without loss in activity, cell extract
Lactobacillus helveticus
MgCl2 and protamine inactivate
Lactobacillus delbrueckii subsp. bulgaricus
MgCl2 and protamine inactivate
Lactobacillus helveticus
no loss in activity after dialysis against Versene
Lactobacillus delbrueckii subsp. bulgaricus
no loss in activity after dialysis against Versene
Lactobacillus helveticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
MgCl2
inactivates
Lactobacillus delbrueckii subsp. bulgaricus
MgCl2
inactivates
Lactobacillus helveticus
additional information
-
Lactobacillus delbrueckii subsp. bulgaricus
additional information
-
Lactobacillus helveticus
protamine
inactivates
Lactobacillus delbrueckii subsp. bulgaricus
protamine
inactivates
Lactobacillus helveticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
particle-bound
-
Lactobacillus delbrueckii subsp. bulgaricus
-
-
particle-bound
-
Lactobacillus helveticus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-[(R)-pantothenoyl]-L-cysteine
Lactobacillus delbrueckii subsp. bulgaricus
-
pantetheine + CO2
-
-
?
N-[(R)-pantothenoyl]-L-cysteine
Lactobacillus helveticus
-
pantetheine + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Lactobacillus delbrueckii subsp. bulgaricus
-
-
-
Lactobacillus helveticus
-
-
-
no activity in Lactobacillus acidophilus
-
-
-
no activity in Lactobacillus arabinosus
-
-
-
no activity in Lactobacillus casei
-
-
-
no activity in Lactobacillus delbrueckii
-
-
-
Storage Stability
Storage Stability
Organism
no loss in activity at 4°C or -20°C, pH 7.0, crude extract, 10-20 mg protein/ml
Lactobacillus delbrueckii subsp. bulgaricus
no loss in activity at 4°C or -20°C, pH 7.0, crude extract, 10-20 mg protein/ml
Lactobacillus helveticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-[(R)-pantothenoyl]-L-cysteine
-
648593
Lactobacillus delbrueckii subsp. bulgaricus
pantetheine + CO2
-
-
-
?
N-[(R)-pantothenoyl]-L-cysteine
-
648593
Lactobacillus helveticus
pantetheine + CO2
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
38
-
soluble preparations stable up to 38°C for 3 h
Lactobacillus delbrueckii subsp. bulgaricus
38
-
soluble preparations stable up to 38°C for 3 h
Lactobacillus helveticus
60
-
rapid loss of activity after 30 min
Lactobacillus delbrueckii subsp. bulgaricus
60
-
rapid loss of activity after 30 min
Lactobacillus helveticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7.5
no sharply defined optimal value
Lactobacillus delbrueckii subsp. bulgaricus
5.5
7.5
no sharply defined optimal value
Lactobacillus helveticus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
rapid loss of activity below pH 5.5 and above pH 7.5, cell extract
Lactobacillus delbrueckii subsp. bulgaricus
5.5
7.5
rapid loss of activity below pH 5.5 and above pH 7.5, cell extract
Lactobacillus helveticus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no requirement
Lactobacillus delbrueckii subsp. bulgaricus
additional information
no requirement
Lactobacillus helveticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no requirement
Lactobacillus delbrueckii subsp. bulgaricus
additional information
no requirement
Lactobacillus helveticus
General Stability (protein specific)
General Stability
Organism
dialysed at 4°C, pH 5.5-7.5, without loss in activity, cell extract
Lactobacillus delbrueckii subsp. bulgaricus
dialysed at 4°C, pH 5.5-7.5, without loss in activity, cell extract
Lactobacillus helveticus
MgCl2 and protamine inactivate
Lactobacillus delbrueckii subsp. bulgaricus
MgCl2 and protamine inactivate
Lactobacillus helveticus
no loss in activity after dialysis against Versene
Lactobacillus delbrueckii subsp. bulgaricus
no loss in activity after dialysis against Versene
Lactobacillus helveticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
MgCl2
inactivates
Lactobacillus delbrueckii subsp. bulgaricus
MgCl2
inactivates
Lactobacillus helveticus
additional information
-
Lactobacillus delbrueckii subsp. bulgaricus
additional information
-
Lactobacillus helveticus
protamine
inactivates
Lactobacillus delbrueckii subsp. bulgaricus
protamine
inactivates
Lactobacillus helveticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
particle-bound
-
Lactobacillus delbrueckii subsp. bulgaricus
-
-
particle-bound
-
Lactobacillus helveticus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-[(R)-pantothenoyl]-L-cysteine
Lactobacillus delbrueckii subsp. bulgaricus
-
pantetheine + CO2
-
-
?
N-[(R)-pantothenoyl]-L-cysteine
Lactobacillus helveticus
-
pantetheine + CO2
-
-
?
Storage Stability (protein specific)
Storage Stability
Organism
no loss in activity at 4°C or -20°C, pH 7.0, crude extract, 10-20 mg protein/ml
Lactobacillus delbrueckii subsp. bulgaricus
no loss in activity at 4°C or -20°C, pH 7.0, crude extract, 10-20 mg protein/ml
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-[(R)-pantothenoyl]-L-cysteine
-
648593
Lactobacillus delbrueckii subsp. bulgaricus
pantetheine + CO2
-
-
-
?
N-[(R)-pantothenoyl]-L-cysteine
-
648593
Lactobacillus helveticus
pantetheine + CO2
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
38
-
soluble preparations stable up to 38°C for 3 h
Lactobacillus delbrueckii subsp. bulgaricus
38
-
soluble preparations stable up to 38°C for 3 h
Lactobacillus helveticus
60
-
rapid loss of activity after 30 min
Lactobacillus delbrueckii subsp. bulgaricus
60
-
rapid loss of activity after 30 min
Lactobacillus helveticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7.5
no sharply defined optimal value
Lactobacillus delbrueckii subsp. bulgaricus
5.5
7.5
no sharply defined optimal value
Lactobacillus helveticus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
rapid loss of activity below pH 5.5 and above pH 7.5, cell extract
Lactobacillus delbrueckii subsp. bulgaricus
5.5
7.5
rapid loss of activity below pH 5.5 and above pH 7.5, cell extract
Lactobacillus helveticus
Other publictions for EC 4.1.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
648593
Brown
Pantothenylcysteine, a precurs ...
Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus helveticus, no activity in Lactobacillus acidophilus, no activity in Lactobacillus arabinosus, no activity in Lactobacillus casei, no activity in Lactobacillus delbrueckii
J. Biol. Chem.
226
651-661
1957
-
-
-
-
-
6
6
-
2
-
-
2
-
6
-
-
-
-
-
-
-
2
2
-
-
-
-
4
-
2
2
-
2
-
-
-
-
-
-
2
-
-
6
-
6
-
-
2
-
-
2
-
-
-
-
-
-
-
2
2
-
-
-
4
-
2
2
-
-
-
-
-
-
-
-
643174
Hoagland
Biosynthesis of coenzyme A fro ...
Rattus norvegicus
J. Biol. Chem.
207
767-773
1954
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648594
Brown
-
N-Pantothenylcysteine as a pre ...
Gluconobacter oxydans
J. Am. Chem. Soc.
75
2782-2783
1953
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-