Literature summary for 4.1.1.28 extracted from

Clark, W.C.; Pass, P.S.; Venkataraman, B.; Hodgetts, R.B.
Dopa decarboxylase from Drosophila melanogaster. Purification, characterization and an analysis of mutants (1978), Mol. Gen. Genet., 162, 287-297.

No PubMed abstract available

Search for more literature for 4.1.1.28

General Stability

General Stability	Organism
three cycles of freezing, -45°C, and thawing have no effect on enzyme activity, in 0.10 M sodium phosphate buffer, pH 7.2, containg 1 mM dithiothreitol and 10% glycerol	Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Drosophila melanogaster
Hg2+	-	Drosophila melanogaster
N-acetyldopamine	-	Drosophila melanogaster
NEM	1 mM, complete inhibition	Drosophila melanogaster
Zn2+	-	Drosophila melanogaster

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Drosophila melanogaster
3.3	-	L-Tyr	-	Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	2 * 54000, SDS-PAGE	Drosophila melanogaster
102000	-	variable porosity acrylamide gel electrophoresis	Drosophila melanogaster
113000	-	sucrose density gradient sedimentation	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Drosophila melanogaster

Source Tissue

Source Tissue	Comment	Organism	Textmining
larva	mature	Drosophila melanogaster	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.07	-	-	Drosophila melanogaster

Storage Stability

Storage Stability	Organism
0°C, 0.05-0.01 M sodium phosphate buffer, pH 7.2, 1 mM dithiothreitol, stable for 6 weeks	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-Dihydroxyphenylalanine	-	Drosophila melanogaster	3-Hydroxytyramine + CO2	-	?
Tyr	-	Drosophila melanogaster	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 54000, SDS-PAGE	Drosophila melanogaster

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	0.0032-0.32 mM, enhances activity greater than 2fold	Drosophila melanogaster

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Release 2023.1 (January 2023)