Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.25 extracted from

  • Lee, E.; Kim, H.; Kim, D.; Kim, Y.; Nam, S.; Kim, B.; Jeon, S.
    Gene expression and characterization of thermostable glutamate decarboxylase from Pyrococcus furiosus (2013), Biotechnol. Bioprocess Eng., 18, 375-381 .
No PubMed abstract available
Search for more literature for 4.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli (DE3) Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.45
-
 L-tyrosine pH 6.0, 75°C Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
KCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
NaBr activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus
NaCl activity is dependent on various salts, such as CaCl2, NaCl, KCl, and NaBr, with an optimum concentration of 400 mM Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Pyrococcus furiosus
41000
-
 SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U1P6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Tyrosine the enzyme also catalyzes the decarboxylation of L-glutamate and L-asparate with higher efficiency. No activity with D-tyrosine Pyrococcus furiosus Tyramine + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 410000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
PF1159
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 85 40°C: about 50% of maximal activity, 85°C: about 50% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half-life: 60 min Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.83
-
 L-tyrosine pH 6.0, 75°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 pH 4.0: about 40% of maximal activity, pH 7.5: about 45% of maximal activity Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate the ennzyme contains pyridoxal 5'-phosphate Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.37
-
 L-tyrosine pH 6.0, 75°C Pyrococcus furiosus