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Literature summary for 4.1.1.25 extracted from
- Gene expression and characterization of a stress-induced tyrosine decarboxylase from Arabidopsis thaliana (2009), FEBS Lett., 583, 1895-1900.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tagged enzyme in Escherichia coli strain M15 | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.745 | - |
L-tyrosine | - |
Arabidopsis thaliana |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE | Arabidopsis thaliana |
| 232000 | - |
His-tagged enzyme, non-denaturing blue native gel electrophoresis | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8RY79 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ agarose column chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Tyrosine | the recombinant protein refuses all other substrates other than L-tyrosine, suggesting TyrDC to be a specific L-tyrosine decarboxylase | Arabidopsis thaliana | Tyramine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 65000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TyrDC | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | - |
Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
at temperatures above 60°C protein activity sharply declines | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | - |
Arabidopsis thaliana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
at pH values above 9.5 the enzymatic activity rapidly declines | Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | salt stress gives rise to a transient decrease of TyrDC mRNA levels by approximately 50% after 60 min | down |
| Arabidopsis thaliana | heat and cold stress, as well as stimulation by pathogens trigger no significant alteration of TyrDC transcripts | additional information |
| Arabidopsis thaliana | TyrDC transcription strongly responds to wounding, TyrDC levels peak within 30 min and transcript levels decline thereafter. TyrDC is rapidly activated by drought stress, reaching maximum levels after 60 min | up |
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