Cloned (Comment) | Organism |
---|---|
transformed into Escherichia coli BL21(DE3) | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 2 mM, complete inhibition | Methanocaldococcus jannaschii | |
O-Methylhydroxylamine | 2 mM, complete inhibition | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
L-tyrosine | - |
Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Methanocaldococcus jannaschii |
90000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q60358 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | 95% of the activity with L-tyrosine | Methanocaldococcus jannaschii | 3-aminopropionic acid + CO2 | - |
? | |
L-glutamate | 80% of the activity with L-tyrosine | Methanocaldococcus jannaschii | 4-aminobutyric acid + CO2 | - |
? | |
L-Tyrosine | - |
Methanocaldococcus jannaschii | Tyramine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MfnA protein | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
10 min, enzyme retains full activity | Methanocaldococcus jannaschii |
110 | - |
10 min, 68% loss of activity | Methanocaldococcus jannaschii |
121 | - |
10 min, complete loss of activity | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Methanocaldococcus jannaschii |