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Literature summary for 4.1.1.23 extracted from

  • Chen, L.; Wei, D.; Zhang, Q.; Yu, X.; Wang, Y.; Zhu, X.
    Orotidine 5'-phosphate decarboxylase-based reusable in situ genetic editing system Development and application in taxol-producing Pestalotiopsis microspora (2015), Eng. Life Sci., 15, 542-549 .
No PubMed abstract available
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Application

Application Comment Organism
molecular biology the orotidine 5'-phosphate decarboxylase encoding gene, pm-ura3, from Pestalotiopsis microspora strain NK17 is utilized as a marker in the construction of a reusable system for gene scarless deletion, restoration, and tagging. No heterogeneous markers are needed, and the accumulation of foreign DNA sequences such as HisG, FRP, and LoxP is avoided Pestalotiopsis microspora

Cloned(Commentary)

Cloned (Comment) Organism
gene pm-ura3, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DB3.1, recombinant expression as genetic marker in Pestalotiopsis microspora strain NK17 via transformation using the Agrobacterium tumefaciens strain LBA4404 system Pestalotiopsis microspora

Protein Variants

Protein Variants Comment Organism
additional information the orotidine 5'-phosphate decarboxylase encoding gene, pm-ura3, from Pestalotiopsis microspora strain NK17 is utilized as a marker in the construction of a reusable system for gene scarless deletion, restoration, and tagging. As a positive marker, gene pm-ura3 can be used to targeted locus of interest in a start uracil auxotrophic host. It then can be replaced by any artificial DNA sequence, e.g. a mutated ORF, under the negative selection of 5-fluoroorotic acid. By this method, genes of interest can be edited in situ and the marker can be recycled. The system is applied to study the function of pm-mus53, it is observed that the gene has little effect on the nonhomologous end-joining process mediated by Agrobacterium tumefaciens-mediated transformation. The expression of a putative taxadiene synthase in taxol biosynthetic pathways of Pestalotiopsis microspora is investigated revealing that it is differentially expressed in solid and liquid medium. Method overview Pestalotiopsis microspora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Pestalotiopsis microspora
-
 UMP + CO2
-
 ?
Orotidine 5'-phosphate Pestalotiopsis microspora NK17
-
 UMP + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pestalotiopsis microspora A0A0A0WDU2
-
-
Pestalotiopsis microspora NK17 A0A0A0WDU2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Orotidine 5'-phosphate
-
 Pestalotiopsis microspora UMP + CO2
-
 ?
Orotidine 5'-phosphate
-
 Pestalotiopsis microspora NK17 UMP + CO2
-
 ?

Synonyms

Synonyms Comment Organism
Orotidine 5'-phosphate decarboxylase
-
 Pestalotiopsis microspora
pm-ura3
-
 Pestalotiopsis microspora