Cloned (Comment) | Organism |
---|---|
gene OMPDC, recombinant expression of His-tagged enzyme in Escherichia coli | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
recombinant His-tagged enzyme, gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Plasmodium falciparum | - |
UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | A0A1D3TG46 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 33000, recombinant His-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
More | structure analysis, homology modeling | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
OMPDC | - |
Plasmodium falciparum |
Orotidine 5'-monophosphate decarboxylase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
metabolism | orotate phosphoribosyltransferase (OPRT) and orotidine 5'-monophosphate decarboxylase (OMPDC) from the human malaria parasite Plasmodium falciparum, the fifth and sixth enzyme of the de novo biosynthetic pathway, form a multienzyme complex. The complex physically exists as heterotetrameric (OPRT)2(OMPDC)2 complex containing two subunits each of OPRT and OMPDC, and has catalytic kinetic advantages over the monofunctional enzyme | Plasmodium falciparum |
additional information | protein-protein interaction in the (OPRT)2(OMPDC)2 enzyme complex by bifunctional chemical cross-linker, liquid chromatography-mass spectrometric analysis and homology modeling, structural models for the protein-protein interaction of the heterotetrameric (OPRT)2(OMPDC)2 multienzyme complex are proposed, allosteric control in protein-protein-interactions | Plasmodium falciparum |