Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 0.1 mM, 2.2fold stimulation | Sulfolobus acidocaldarius | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.1 mM, at 70°C, 2.2fold activation | Sulfolobus acidocaldarius | |
6-Azauracil | 1 mM, 1.2fold activation | Sulfolobus acidocaldarius | |
N-ethylmaleimide | 10 mM, at 70°C, 17.8fold activation | Sulfolobus acidocaldarius | |
N-ethylmaleimide | 10 mM, 18fold stimulation | Sulfolobus acidocaldarius | |
Uracil | 1 mM, 1.3fold stimulation | Sulfolobus acidocaldarius |
General Stability | Organism |
---|---|
orotidine 5'-phosphate and UMP stabilize | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorouracil | 1 mM, 14% inhibition | Sulfolobus acidocaldarius | |
Dimethylsulfoxide | 20% v/v, 8% inhibition | Sulfolobus acidocaldarius | |
EDTA | 10 mM, 6% inhibition; 10 mM, at 70°C, weak inhibition | Sulfolobus acidocaldarius | |
Guanidine-HCl | 2.0 M, complete inhibition | Sulfolobus acidocaldarius | |
Guanidinium chloride | 1 M, at 70°C, 81.4% inhibition | Sulfolobus acidocaldarius | |
phosphate | low activity in phosphate buffers, inhibitory or destabilizing effect | Sulfolobus acidocaldarius | |
SDS | 1%, at 70°C, complete inactivation; 1%, complete inhibition | Sulfolobus acidocaldarius | |
UMP | 1 mM, 19% inhibition; 1 mM, at 70°C, 19% inhibition | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, SDS-PAGE | Sulfolobus acidocaldarius |
46000 | - |
gel filtration | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Sulfolobus acidocaldarius | - |
UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | Sulfolobus acidocaldarius DG6 / ATCC 49426 | - |
UMP + CO2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | at 70°C, the enzyme is inactivated by guanidinium chloride at 1 M or higher concentrations | Sulfolobus acidocaldarius |
SDS | at 70°C, the enzyme is inactivated by 1% SDS or higher concentrations | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
- |
- |
Sulfolobus acidocaldarius DG6 / ATCC 49426 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
1420fold | Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0124 | - |
pH 8.0, 70°C | Sulfolobus acidocaldarius |
12.4 | - |
pH 8.5, 70°C | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Sulfolobus acidocaldarius | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Sulfolobus acidocaldarius DG6 / ATCC 49426 | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22000, SDS-PAGE | Sulfolobus acidocaldarius |
homodimer | 2 * 22000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
Orotidine-5'-monophosphate decarboxylase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Sulfolobus acidocaldarius |
98 | - |
- |
Sulfolobus acidocaldarius |
98 | - |
above | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable | Sulfolobus acidocaldarius |
85 | - |
or above, extremely thermostable in presence of substrate or product, considerable thermal inactivation in their absence | Sulfolobus acidocaldarius |
85 | - |
in the absence of substrate and product, however, a considerable thermal inactivation was observed at 85°C or above | Sulfolobus acidocaldarius |
90 | - |
10 min, 90% loss of activity | Sulfolobus acidocaldarius |
90 | - |
10 min, over 90% loss of activity, in absence of substrate and product | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
broad pH-optimum centered about pH 8 | Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Sulfolobus acidocaldarius |
8.5 | - |
assay at | Sulfolobus acidocaldarius |